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  • Author or Editor: L. P. Stoltz x
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Abstract

A combination of 1.0 mg/liter 6-benzylamino purine (BA) and 0.1 mg/liter naphthaleneacetic acid (NAA) with 3% sucrose produced the greatest number of shoots from sterile leaf pieces of tarrogon (Artemesia dracunculus L. var. sativa) placed on Linsmaier-Skoog (LS) medium. Plantlet regeneration also occurred with 0.2 mg/liter BA without auxin and with 2% sucrose.

Open Access
Authors: and

Abstract

Effects of a wet tent (cloth continuously wetted), an intermittent mist, and a shade system were examined for establishment, survival, and growth of microcuttings of three cultivars of Euphorbia fulgens Karw. ex Klostch. The wet tent system resulted in high survival rates and rapid plantlet growth. Microcuttings placed in the mist system had survival rates close to that in the wet tent, but had the poorest plantlet growth. Microcuttings placed directly in the shade had a poor survival rate, but plantlets that survived grew as well as those from the wet tent. In all three acclimatization systems, microcuttings of the white cultivar had the highest survival rate; those of the orange and red cultivars were second and third, respectively. No difference was found in plantlet growth among the surviving cultivars after removal from treatment in acclimatization systems. Microcuttings 41 to 50 mm long survived best; survival rate increased with microcutting length. The minimum microcutting length for satisfactory survival rates (79%) was 31 mm. Microcuttings rooted a month earlier than did conventional stem-tip cuttings in the wet-tent system.

Open Access
Authors: and

Abstract

Effects of trans-zeatin, sucrose, myo-inositol, and medium pH on shoot proliferation of Euphorbia fulgens Karw. ex Klostch were studied in vitro. Maximum shoot production occurred on media supplemented with 5 μΜ zeatin, but maximum shoot length with 5 to 15 μΜ. Shoot production increased with sucrose concentration, and was maximal at 131.5 mM. myo-lnositol concentration up to 0.6 mM did not have a significant effect on shoot production, but >1.1 mM reduced it. The optimal medium pH was 5.3 for shoot proliferation, but lower pH values stimulated shoot growth. Chemical names used: trans-2-methyl-4-(1H-purin-6-ylamino)-2-buten-l-ol (trans-zea-tin), α-D-glucopyranosyl-β-D-fructofuranoside (sucrose).

Open Access

Abstract

A method is described for determining glucose metabolism by the C-6/C-1 ratio. After an initial lag period to allow for equilibration of labelled glucose, an increase of accumulated 14CO2 in rose petals was linear with time. By taking the limit of the ratio of the C-1 and C-6 lines, one can arrive at the C-6/C-1 ratio as a ratio of the slopes of the lines. The slope of each of the lines can be calculated with 2 determinations within a relatively short time period. This method reduces metabolic recycling of the glucose and other problems associated with the traditional method of determining the C-6/C-1 ratio.

Open Access

Abstract

The pentose phosphate shunt (PPS) was shown to be active in roses (Rosa hybrida L.). The C6/C1 ratio indicated that possibly as much as 50% of the glucose oxidized in the rose flower is through the PPS. However, the activity of the PPS in relation to the Embden-Meyerhof-Parnas pathyway-tricarboxylic acid cycle (EMP-TCA) pathway did not change significantly throughout the cut life of the rose. There was a drop in the respiratory rate of petals throughout the cut flower life. The inner petals exhibited a consistantly higher respiratory rate than did outer petals regardless of whether the flower was kept in water or preservative.

Open Access

Abstract

Nodal sections of actively growing apical shoots from greenhouse-grown plants of Euphorbia fulgens Karw. ex Klotsch initiated new shoots after 4 weeks on a modified Murashige and Skoog (MS) medium supplemented with 9.1 μm zeatin. When cultures from the initiation stage were transferred for proliferation to the same medium, up to 14 shoots 5 mm long or longer were obtained per culture 4 weeks later. Through subcultures, 40 transplantable shoots per explant could be produced within 12 weeks. Shoots were rooted in vitro in the greenhouse with satisfactory survival rates. Chemical names used: (E)-2-methyl-4-(1H-purin-6-ylamino)-2-buten-1-ol (zeatin).

Open Access

Abstract

Backcrosses and outcrosses of mite resistant strawberry selections and combinations of resistant selfed lines were made. Backcrosses to the resistant parents resulted in intermediate progeny means but they were closer to that of the less resistant parent. Backcrosses to the susceptible parent also resulted in intermediate progeny means but they were close to that of the more resistant parent. Outcrosses gave progenies similar to those of backcrosses but with less spread in resistance. Crosses of certain resistant selfed selections showed almost complete dominance for mite resistance.

Open Access

Abstract

Five clones of strawberries varying in degree of resistance to the two-spotted spider mite, Tetranychus urticae Koch, were grown at four nutrient levels. Foliage samples were analyzed for minerals, amino acids, total sugar and total starch. The plants were infested with this mite and were rated for injury. Nitrogen of clones and treatments showed significant correlation with mite injury at the 0.05 and 0.01 levels respectively. Phosphorus of clones was significantly correlated with mite injury. Mite injury and treatments, but not clones, were significantly correlated when the amino acids classified as non-essential to Tetranychus urticae were considered. Total starch and total sugar of treatments had significant negative correlations with mite damage.

Open Access

Abstract

A higher level of auxin-like growth promoter was found in seedlings of the tall cucumber cultivar than in those of the dwarf. However, the highest level of GA-like growth promoter was found in the shoot tips of the dwarf cultivar, both in seedlings and mature plants; and this fraction was chromatographically similar to authentic GA3. The difference in GA-like activity between dwarf and tall was greater in the shoot tips of seedlings than in those of mature plants. Furthermore, the content of GA-like material was similar in dark grown tall and dwarf cucumber, but in light grown seedlings the activity was nearly twice as high in the dwarf as in the tall. These data are supported by mevalonate-2-14c feeding experiments.

Open Access