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  • Author or Editor: L. Laverdière x
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One hundred twenty-eight field samples of 25 potato stem sections were analyzed for detecting bacterial ring rot (BRR). Samples containing more than 105, between 105 and 104, and <104 immunofluorescing BRR cells per milliliter of sample, detected using immunofluorescence-antibody staining with MAb9A1, were used to compare the efficiency of two other detection methods. Samples were screened with a digoxigenin-labeled DNA probe (Bh1 2, 0.6 kbp) detected by chemioluminescence on nylon membrane. Samples also were screened with a PCR test using primers derived from the sequence of the Bh12 probe. DNA probe tests on these three bacterial concentrations showed a detection efficiency of 100%, 76.8%, and 8.0%, respectively, whereas detection efficiencies of 100%, 100%, and 84.5% were obtained with PCR tests. Almost all positive samples gave the expected 403 bases ethidium-bromide-stained band when the amplified products were analyzed on 1.4% agarose gel. Thus, the PCR test was a sensitive detection method for screening bacterial ring rot of potato.

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