There are economic and knowledge-based challenges that must be addressed for indoor farms to be viable in the United States despite their potential benefits. A mixed-methods approach was used to identify the needs of specialty crop growers and stakeholders interested in or currently using indoor propagation environments to grow seedlings, cuttings, and tissue-cultured plants. An online survey evaluated specialty crop growers’ current use of indoor plant propagation environments and their needs related to indoor plant propagation. A focus group was then conducted to further understand the needs for indoor plant propagation by stakeholders. Industry participants were largely motivated to adopt indoor propagation environments to reduce crop losses (“shrinkage”), increase productivity per unit of land area, ensure faster germination or rooting, improve plant quality, and profit from anticipated economic benefits. Research and education priority areas identified by stakeholders included economic costs and benefits (including capital investment and energy costs), improved crop quality, production time, uniformity, reduced shrinkage, and strategies to improve light management indoors. Based on the results, research efforts must determine and prioritize the most important economic considerations and production advantages to fill important gaps in knowledge about indoor plant propagation.