The physiological and physico-chemical responses of jute, a tropical green leafy vegetable, to several temperature conditions were determined during postharvest storage. Jute were sourced from wholesale market and harvested from the university farm, packed in low-density polyethylene bags and stored at 1 to 30 °C and 1 to 20 °C, respectively, before it was analyzed for postharvest quality changes. There was no significant difference in the time-temperature tolerance of both leaves in all treatments. At 1 °C and 8 °C, both jute showed high sensitivity to chilling injury, which manifested by browning of the stems, darkening of young and mature leaves, wilting, and excretion of slimy substances. These symptoms developed within 3 to 9 days at 1 °C and 5 to 13 days at 8 °C, and it seems related with the decrease of ortho-diphenol content. The chlorophyll fluorescence of jute measured in terms of Fv: Fmax ratio decreased before the onset of browning and remained at lower levels during development of chilling injury at 1 and 8 °C. The ethylene concentration decreased after increasing for 1 day at 8 °C and before chilling injury occurred at 1 °C. However, at 15, 20, and 30 °C, both jute were more susceptible to yellowing with chlorophyll degradation and abscission of leaves due to senescing effect of high-temperature storage. Senescent symptoms were evident within 1 to 5 days at 30 °C and 3 to 7 days at 15 and 20 °C. Jute with stems partly immersed in water during storage had lower respiration rates at 1 and 8 °C due to its sensitivity to chilling injury manifested by wilting of leaves within 1 to 4 days of storage.