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Intergenomic F1 hybrids between L. auratum and L. henryi were made and backcrossed to Oriental hybrids to produce BC1 progenies. The F1 intergenomic hybrids produced a relevant frequency of 2n-gamete and fertile pollen. Analyses of pollen size, viability, and germination ability were checked under microscopic observation. GISH analysis confirmed that F1 intergenomic hybrids possess a set of chromosomes from both parents and showed an intermediate morphological phenotype. Twenty BC1 plants were generated by in vitro embryo rescue technique, and analyzed for genome composition by GISH. All plants were triploid, showing 12 from female parent (2× Oriental) and 24 from male (2× F1 hybrid). Based on the GISH analyses, 19 BC1 plants were derived from FDR 2n-gamete and only one plant was derived from IMR 2n-gamete of F1 hybrid. The genome composition of 19 BC1 plants from FDR 2n-gamete possess 12 Orientals + 12 L. auratum + 12 L. henryi chromosomes with some extent of homoeologous recombination between L. auratum and L. henryi. However, one plant from IMR 2n-gamete origin contains an odd number of parental chromosomes from F1 hybrid, showing 12 Oriental + 14 L. auratum + 10 L. henryi chromosomes. In this case, two L. auratum chromosomes recombinant with L. henryi chromosome segments were added and L. henryi counterpart chromosomes were deleted, respectively.
The effects of watering frequency, bulb size, temperature, and Promalin on flowering of Cyrtanthus elatus, were investigated. Bulbs must be 8 cm, in circum., with minimum of 10 leaf bases before the first inflorescence will develop from a swollen leaf base. One inflorescence was initiated for each set of 5 leaf bases. Watering frequencies did not affect flower bud development and abortion. Greater than 73% of plants developing from bulbs larger than 15.3 cm in circum. produced an inflorescence with 4 to 6 flowers when grown at 26/23C (D/N) and sprayed with 3,000 ppm Promalin. Anthesis was accelerated from 64 days to 39 days when temperatures were increased from 16/13 to 26/23C. A second inflorescence was also produced from some Promalin-treated plants grown at 26/23C. Flower bud abortion of inflorescences formed on the outer row of leaf bases appeared to contribute to irregular flowering.