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The design of a type of drainage lysimeter, as tested with trees of Pyrus serotina Rehder var. culta Rehder `Hosui' is described. All lysimeter operations and monitoring of irrigation and drainage volumes were managed by a “multi-tasking” controller/datalogger. It was possible to apply different irrigation levels to each of three sets of four random lysimeters. Evapotranspiration (ET) was calculated using a conservation of water equation, with differences between irrigation inputs and drainage outputs corrected for changes in soil-water content. ET ranged between 3.3 and 10.7 liters/tree per day in well-watered, noncropped trees in late Summer and Fall 1990. These rates correspond to ET of 0.13 to 0.43 liter·cm-2·day-1 and 0.96 to 3.10 liters·m-2·day-1 on trunk cross-sectional area and canopy area bases, respectively. The correlation coefficient between ET and Class A pan evaporation was >0.9 during this period. Weekly crop coefficients for the well-watered trees averaged 0.52 when calculated on a canopy-area basis. When irrigation was withheld for 18 days, the crop coefficient declined to 0.38. There were no differences in ET between trees growing in the two soil profiles, despite significant differences in soil water distribution.
Zoysiagrass (Zoysia sp.) is used as a warm-season turfgrass for lawns, parks, and golf courses in the warm, humid and transitional climatic regions of the United States. Zoysiagrass is an allotetraploid species (2n = 4x = 40) and some cultivars are known to easily self- and cross-pollinate. Previous studies showed that genetic variability in the clonal cultivars Emerald and Diamond was likely the result of contamination (seed production or mechanical transfer) or mislabeling. To determine the extent of genetic variability of vegetatively propagated zoysiagrass cultivars, samples were collected from six commercially available zoysiagrass cultivars (Diamond, Emerald, Empire, JaMur, Meyer, Zeon) from five states (Arkansas, Florida, Georgia, North Carolina, Texas). Two of the newest cultivar releases (Geo and Atlantic) were to serve as outgroups. Where available, one sample from university research plots and two samples from sod farms were collected for each cultivar per state. Forty zoysiagrass simple sequence repeat (SSR) markers and flow cytometry were used to compare genetic and ploidy variation of each collected sample to a reference sample. Seventy-five samples were genotyped and an unweighted pair group method with arithmetic mean clustering revealed four groups. Group I (Z. japonica) included samples of ‘Meyer’ and Empire11 (‘Empire’ sample at location #11), Group II (Z. japonica × Z. pacifica) included samples of ‘Emerald’ and ‘Geo’, Group III (Z. matrella) included samples of ‘Diamond’ and ‘Zeon’, and Group IV (Z. japonica) consisted of samples from ‘Empire’, ‘JaMur’, ‘Atlantic’, and Meyer3 (‘Meyer’ at sample location #3). Samples of ‘Empire’, ‘Atlantic’, and ‘JaMur’ were indistinguishable with the markers used. Four samples were found to have alleles different from the respective reference cultivar, including two samples of ‘Meyer’, one sample of ‘Empire’, and one sample of ‘Emerald’. Three of these samples were from Texas and one of these samples was from Florida. Three of the four samples that were different from the reference cultivar were university samples. In addition, one sample, Empire11, was found to be an octoploid (2n = 8x = 80). For those samples that had a fingerprint different from the reference cultivar, contamination, selfing, and/or hybridization with other zoysiagrasses may have occurred.