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  • Author or Editor: Kazuki Moriguchi x
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Primordial leaves of Japanese persimmon (Diospyros kaki L. cv. Jiro) were excised from dormant buds and cultured on Murashige and Skoog medium with the nitrates reduced to half strength (MS½N) and supplemented with 0.1 to 10.0 µm zeatin + 0.1 to 10.0 µm IAA. After 40 days of culture in the dark, yellowish-white calli were formed at 10.0 µm zeatin + 1.0 µm IAA. When these calli were transferred to MS½N medium supplmented with zeatin (1.0 to 10.0 µm) + IAA (0 to 10.0 µm), 24% of them produced adventitious buds at 10.0 µm zeatin + 0.1 µm IAA under a 12-hr photoperiod. Further shoot growth occurred on the same medium without IAA. Basal ends of the shoots were quick-dipped in 1.25 mm IBA and placed on half-strength MS½ N medium. Nearly two-thirds of the dipped shoots rooted within 30 days. Plantlets regenerated in vitro were then transferred to a mixture of 1 peat: 1 perlite: 1 vermiculite and acclimatized for potting. Chemical names used: 1H-indole-3-acetic acid (IAA); 1H-indole-3-butyric acid (IBA); (E)-2-methyl-4-(1-H-purin-6-ylamino)-2-buten-1-o1 (zeatin).

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