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- Author or Editor: Kathryn R. Kleiner x
A greenhouse study was designed to determine the relative heat tolerance of 10 lima bean cultivars and to evaluate the effects of high temperature on lima bean yield. Cultivars were arranged in a randomized complete block with three blocks per treatment. The temperature treatments were 25C day/15C night and 35C day/25C night. Cultivars varied in their response to the higher temperature, allowing for classification into three heat response groups: intolerant, average, and tolerant. Heat-intolerant plants did not experience a significant reduction in number of pods, but number of beans and total bean weight were reduced at the higher temperature. Number of seeds per pod and average weight per bean also tended to decrease in intolerant plants at 35C. In future experiments, these data will be correlated with random amplified DNA (RAPD) markers. These markers will be evaluated for their potential for heat tolerance screening.
Lima beans are an important vegetable crop to the processing industry in Delaware, but yields in Delaware are below other areas due to heat. The objective was to correlate RAPD markers from heat-tolerant and intolerant cultivars with phenotypic data. Twenty-five primers were used, 10 of which generated 25 polymorphic bands among 11 cultivars. MDS analysis of genetic distance among the cultivars shows segregation into two major clusters, with Kingston as a distant outlier. Kingston's position can be correlated to published data reporting its consistently good yields even when temperatures are high. The results of this study indicate RAPD markers may be used to screen for cultivars that have high yield potentials despite high temperatures. Further studies to screen F, and inbreeds will determine the usefulness of these markers in breeding programs.