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- Author or Editor: Karen Lee Prouty x
Abstract
Protoplasts were isolated from potato Solanum tuberosum L. leaves (‘Russet Burbank’) and plated in cell layers containing either BA, kinetin, or 2iP at concentrations of 0, 0.005, 0.05, or 5.0 mg·liter−1. After 17 days in culture, plating efficiencies were determined and BA at the 0.5 and 0.05 mg· liter−1 concentrations was associated with significantly higher plating efficiencies than the other two sources. The methodology for following development of individual protoplasts is discussed. Chemical names used: N-(phenylmethyl)-1H-purin-6-amine (BA); N-(2-furanylmethyl)-1H-purin-6-amine (kinetin); and N-(3-methyl-2-butenyl)-1H-purin-6-amine (2iP).
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