Recently, claims have been made that the use of glyphosate and transgenic crop traits increases plant susceptibility to pathogens. Transgenic traits used widely for years in dent corn are now available in commercial sweet corn cultivars, specifically, the combination of glyphosate resistance (GR) and Lepidoptera control (Bt). The objective was to assess the interactions of the GR+Bt trait, glyphosate, and Goss’s wilt on sweet corn. Nine treatments were tested under weed-free conditions at two sites in 2013 and 2014. Treatments included two isogenic cultivars differing only in the presence or absence of GR+Bt, with and without postemergence application of glyphosate, and inoculation with the causal agent of Goss’s wilt (Clavibacter michiganensis ssp. nebraskensis) before glyphosate application, after glyphosate application, or no inoculation. Results failed to show glyphosate or the GR+Bt trait influenced sweet corn susceptibility to Goss’s wilt. The only factor affecting Goss’s wilt incidence was whether plants were inoculated with C. michiganensis ssp. nebraskensis. In the absence of glyphosate application, yet under weed-free conditions, several yield traits were higher in sweet corn with the GR+Bt trait. Results showed that the GR transgene confers the same level of tolerance to glyphosate in sweet corn as observed previously in dent corn. If true, recent claims about glyphosate and transgenic traits increasing plant disease would be of major concern in sweet corn; however, no relationships were found between the GR+Bt trait and/or glyphosate to Goss’s wilt incidence in sweet corn.