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Josh A. Honig, Stacy A. Bonos and William A. Meyer

Kentucky bluegrass (Poa pratensis L.) is an important facultative apomictic temperate perennial grass species used for both forage and cultivated turf. Through apomixis, this species is able to propagate diverse and odd ploidy levels, resulting in many genetically distinct phenotypes. A wide range of diverse cultivars and accessions of kentucky bluegrass have been previously characterized based on common turf performance or morphological characteristics as well as by random amplified polymorphic DNA (RAPD) markers. Although previous characterization efforts have provided valuable information, the use of both morphological characteristics and RAPD markers for genetic diversity analysis has limitations. In the current report, we developed and characterized 88 novel microsatellite markers for kentucky bluegrass. Polymorphism for each marker was assessed in 265 kentucky bluegrass cultivars, experimental selections, collections, and hybrids. The number of alleles for individual microsatellites ranged from four to 81 with an average of 38.3 alleles per simple sequence repeat. These polymorphic microsatellite markers would be useful tools for investigating genetic diversity, creation of genetic linkage maps, assessment of levels of apomixis in cultivars and experimental varieties, and identification of aberrant progeny in apomictic kentucky bluegrass breeding programs.

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Josh A. Honig, Vincenzo Averello, Stacy A. Bonos and William A. Meyer

Kentucky bluegrass (Poa pratensis L.) is an important facultative apomictic temperate perennial grass species used for both forage and cultivated turf. Through apomixis, this species is able to propagate diverse and odd ploidy levels, resulting in many genetically distinct phenotypes. A wide range of diverse cultivars and accessions of kentucky bluegrass have been previously characterized based on pedigree, common turf performance, and morphological characteristics to create a kentucky bluegrass cultivar classification system. The objectives of the current study were to assess the amount of genetic divergence among kentucky bluegrass cultivars, experimental selections, and plant collections and revise/update the original pedigree, turf performance, and morphological characteristics kentucky bluegrass classification system using recently described kentucky bluegrass microsatellite [simple sequence repeat (SSR)] markers. In this study, 247 kentucky bluegrass cultivars, experimental selections, and collections were genotyped using 25 SSR markers. SSR markers showed a strong correlation between genetic relatedness as assessed by molecular markers and the original kentucky bluegrass classification system and also provided justification for a revision/update of the classification system. Traditional classification types that were supported by the current SSR analysis include BVMG, Compact, Compact-America, Julia, Mid-Atlantic, Midnight, and Shamrock types. Newly proposed classification types included Cynthia, Jefferson/Washington, Limousine, P-105, Sydsport, and three Eurasian types. The majority of cultivars, experimental selections, and collections were uniquely identified with the current set of SSR markers. Genetic relationships of individuals as assessed by SSR markers closely matched known pedigrees. The current set of SSR markers can be used to rapidly genotype and assign new cultivars/accessions to kentucky bluegrass classification types and assess genetic relatedness among individuals and should be considered for use in a kentucky bluegrass plant variety protection program.

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Megan F. Muehlbauer, Josh A. Honig, John M. Capik, Jennifer N. Vaiciunas and Thomas J. Molnar

The development of new cultivars resistant to the disease eastern filbert blight (EFB), caused by Anisogramma anomala, is of primary importance to hazelnut (Corylus sp.) breeders in North America. Recently, a large number of EFB-resistant cultivars, grower selections, and seedlings from foreign germplasm collections were identified. However, for a significant number of these, little is known of their origin, relationships, or genetic background. In this study, 17 microsatellite markers were used to investigate the genetic diversity and population structure of 323 unique accessions, including EFB-resistant and tolerant germplasm of uncertain origins, in comparison with a panel of known reference accessions representing a wide diversity of Corylus cultivars, breeding selections, and interspecific hybrids. The resulting allelic data were used to construct an unweighted pair group method using arithmetic averages (UPGMA) dendrogram and STRUCTURE diagram to elucidate relationships among the accessions. Results showed 11 consensus groups with EFB-resistant or tolerant accessions in all, providing strong evidence that EFB resistance is relatively widespread across the genus Corylus. Furthermore, open-pollinated seedlings tended to group together with reference accessions of similar geographic origins, providing insight into their genetic backgrounds. The results of this study add to the growing body of knowledge of hazelnut genetic resources and highlight recently introduced EFB-resistant seedling germplasm as new, unrelated genetic pools of resistance.

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John M. Capik, Megan Muehlbauer, Ari Novy, Josh A. Honig and Thomas J. Molnar

Stable genetic resistance to the fungal disease eastern filbert blight (EFB), caused by Anisogramma anomala, is vital for sustainable production of European hazelnut (Corylus avellana) in eastern North America. In this study, new hazelnut germplasm from the Russian Federation, Ukraine, and Poland (a total of 1844 trees from 66 seed lots) was subjected to A. anomala under field conditions over at least five years in New Jersey. Plants were then rated for the presence of EFB using an index of 0 (no disease) through 5 (all stems containing cankers). Nuts of the resistant trees were evaluated to identify plants with improved kernel characteristics. Genomic DNA of these trees was also screened with sequence-characterized amplified region (SCAR) markers generated by the primers BE-03, BE-33, and BE-68, which are closely linked to the single dominant R-gene of ‘Gasaway’, to assess the resistant seedlings for the presence of this well-known source of resistance. At final evaluation, 76 trees remained free of disease with nine expressing only minor symptoms (rating 1 or 2). The resistant trees spanned 24 different seed lots representing all three countries. The remaining trees ranged from moderately to severely infected with 81% of the total collection rating 5. Several of the resistant trees were found to produce commercial-sized (≈12 mm diameter), round kernels that blanched well. Although the results of the ‘Gasaway’ SCAR primers were inconclusive, the diverse collection origins and disease phenotypes provide evidence that novel sources of resistance were likely identified in this study. These new plants should broaden the genetic base of EFB-resistant C. avellana hazelnut germplasm available for breeding.

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Jennifer Johnson-Cicalese, James J. Polashock, Josh A. Honig, Jennifer Vaiciunas, Daniel L. Ward and Nicholi Vorsa

Fruit rot is the primary threat to cranberry (Vaccinium macrocarpon) production in the northeastern United States, and increasingly in other growing regions. Efficacy of chemical control is variable because the disease is caused by a complex of pathogenic fungi. In addition, cranberries are often grown in environmentally sensitive areas, placing restrictions on chemical control measures. Thus, a major focus of the cranberry breeding program is to develop cultivars with improved fruit rot resistance (FRR). Several genetically diverse sources of FRR have been identified in our germplasm collection. However, the most resistant accessions lack one or more attributes; e.g., productivity, required for commercial acceptance. These resistant accessions were used in crosses with elite high-yielding selections and in 2009, 1624 progeny from 50 crosses were planted in 2.3-m2 field plots. During 2011–13, under field conditions with very limited fungicide management, disease pressure was severe, allowing evaluation for FRR. Plots were rated on a 1–5 scale for incidence of fruit rot (where 1 = 0% to 20% rot and 5 = 81% to 100% rotted fruit), and rotted fruit counts were made from selected plots to validate the ratings. There was a good correlation in the ratings between years (2011 vs. 2012: r = 0.59, P < 0.0001; 2011 vs. 2013: r = 0.50, P < 0.0001; 2012 vs. 2013: r = 0.62, P < 0.0001), and between rot ratings and percent rotted fruit (r = 0.90, P < 0.0001). Significant differences were found between and within families for FRR. High heritability estimates (h 2 = 0.81) were obtained with midparent-offspring regression of mean fruit rot ratings, indicating additive genetic variance for FRR. Introgression of FRR into higher yielding genetic backgrounds was also accomplished, as some progeny exhibiting high FRR also had commercially viable yield (>300 g/0.09 m2), as well as good berry size and color. Selections are being further evaluated for potential cultivar release.