Search Results

You are looking at 1 - 2 of 2 items for

  • Author or Editor: Josephina G. Niederwieser x
Clear All Modify Search

The physiological stage of donor plants determined to a great extent the morphogenic potential of Lachersalia (Jacq.) hybrid leaves, but the optimal stage for various cultivars was different. Contact of the bud-forming adaxial epidermal cells with the medium did not significantly stimulate in vitro bud formation on Lachenalia leaf explants, but resulted in the formation of callus from the buds of certain hybrids. Wounding on either the adaxial or the abaxial side of leaves had a stimulating effect on certain hybrids, but others did not respond significantly. A reduction in the length of explants from 10 to 3.3 mm resulted in an increase in the total number of buds formed by a specific amount of explant tissue (width of explant = 15 mm).

Free access

Techniques are described to determine whether embryos are formed in ovules of incompatible crosses between Ornithogalum (L.) plants, and to rescue embryos in cases where the development of embryos is halted following fertilization. By using Herr's clearing liquid, it can be ascertained within 5 hours whether hybrid embryos have been formed. Such embryos can be rescued by culturing them in ovulo on basal medium containing 70 g sucrose/liter and no added growth regulators. The embryos' requirement for sucrose changes as they develop; therefore, cultured ovules are transferred after 14 days to a medium containing 10 g sucrose/liter, where germination occurs.

Free access