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The bacterium Xanthomonas campestris pv. campestris (Xcc), causal agent of black rot disease in crucifers was tagged with the luciferase gene complex of the marine bacterium Vibrio fisheri. The growth of the bioluminescent strain in plants and the environment can be monitored by its light emissions. Susceptible cabbage plants were either mist, wound or debris inoculated in the field, soil was inoculated with debris or with suspension culture of genetically engineered Xcc. Plant, soil and air samples will be taken at biweekly intervals to monitor the spread of the bioluminescent bacterium within as well as outside the environmental release site. The transfer of exotic DNA to other bacteria in the environment will also be studied.
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