Search Results
Seeds of Primula acaulis (L.) J. Hill `Dukaat Helderrose' were imbibed for 48 h at 18C, and Maltrin 600 solution was used to separate them into density classes of 1.10, 1.12, 1.14, 1.16, and 1.18 g·cm-3. Seeds were rinsed, air-dried for 48 h at 22C, and germinated at 18C; 80% were separated into density classes between 1.16 and 1.18 g·cm-3. Percentages of germination and high-vigor seedlings were significantly greater for seeds with densities >1.14 g·cm-3. Soluble protein concentration increased with density, whereas insoluble protein concentration was unchanged. Distinct protein groups with estimated molecular weights of ≈50, 33, and 25 kDa were present in the protein profile of nonseparated imbibed seed. These proteins were most abundant at densities ≥1.16 g·cm-3. The 25-kDa protein was present only in higher density seed. Presence of an additional 14-kDa protein was noted in the insoluble protein profile. Certain proteins in the soluble protein fraction maybe used to test for seed vigor.