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Abstract
Tannin cells from fruit of Japanese persimmon (Diospyros kaki L.) cvs. Fuyu [pollination-constant and nonastringent (PCNA)], Chokenji [pollination-variant and nonastringent (PVNA)], Hiratanenashi [pollination-variant and astringent (PVA)], and Kuramitsu [pollination-constant and astringent (PCA)], were observed by florescence microscope (FM) and scanning electron microscope (SEM) on 4 July, when all cultivars were very astringent, and on 5 Sept., when ‘Fuyu’ and ‘Chokenji’ fruit had completely lost their astringency. ‘Hiratanenashi’ and ‘Kuramitsu’ fruit, however, were still quite astringent on the latter date. FM observations on 4 July indicated that tannin cells of all fruit possessed some discontinuous portions in the cell walls. SEM observations of fractured surfaces of fruit flesh verified the existence of pores in the tannin cell walls. Moreover, coagulated internal contents of tannin cells (caused by the fixatives) had protruded through the pores. On 5 Sept., however, the pores in tannin cell walls of ‘Fuyu’ and ‘Chokenji’ were not present, and the surface of coagulated internal contents had become smooth. ‘Hiratanenashi’ and ‘Kuramitsu’ fruit on 5 Sept. showed little change in the structure from that observed on 4 July. Pore occlusion occurred in ‘Hiratanenashi’ fruit that were treated on the tree with ethanol fumes to remove astringency, which indicates that loss of astringency induces structural changes in tannin cell walls.
Abstract
Morphological changes in tannin cells were observed in ‘Fuyu’ [pollination-constant and nonastringent (PCNA)] and ‘Hiratanenashi’ [pollination-variant and astringent (PVA)], two of the four types of Japanese persimmon fruit (Diospyros kaki L.). Pores in the tannin cell walls of ‘Fuyu’ started to occlude on 24 July when cell enlargement had ceased. This occlusion coincided with cessation of tannin accumulation, as determined by soluble tannin content and fresh weight of fruit. The pores were almost completely occluded on 7 Aug. Pore occlusion preceded the coagulation of tannins. In ‘Hiratanenashi’, pores in the tannin cell walls expanded until 7 Aug. When enlargement of tannin cells ceased on 14 Aug., occlusion of the pores in the cell walls was initiated. This event also nearly coincided with cessation of tannin accumulation. The process of occlusion was much slower than in ‘Fuyu’ and was about complete on 16 Oct. Thus, the pores in the tannin cell walls appear to be involved in tannin accumulation.
Abstract
Transpiration rate, stem diameter, and leaf temperature of satsuma mandarin (Citrus unshiu Marc.) were found to be sensitive indicators of stomatal response to ozone exposure. Time of reaction of each to 1.2 ppm ozone was 3 min. Stem diameter showed marked oscillation and leaf temperature slight oscillation, when ozone was applied. Correlation coefficiennt between ozone concentration and transpiration rate at the equilibrium status after the beginning of exposure was r = 0.718, and increasing the concentration of ozone decreased stomata size. Thus, it was determined that low sensitivity of satsuma mandarin to ozone is due to rapid stomatal closure by ozone.