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  • Author or Editor: Jing Wu x
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The art of pressed flowers is a method of artistic expression involving the pressing of flowers, leaves, and other plant organs for artistic creative purposes. However, the pressing process often results in color variation of the plant material, which significantly diminishes the quality of artistic works and must be solved using appropriate techniques. During this research, phenylalanine (10 mmol⋅L−1) was used to treat the petals of postharvest Petunia flowers to investigate the impact of phenylalanine on mitigating color variation, and the effect of phenylalanine on inhibiting the color variation of Petunia petals during the pressing process was evaluated by color measurement, physicochemical indices, and gene expression level analyses. Using the CIEL*a*b* color measurements, the samples from the test group had significantly higher brightness (L*) and red coloration (a*) at the final stage (S4) than the control group. In addition, phenylalanine had a significant inhibitory effect on malondialdehyde and superoxide anion accumulations in Petunia petals during pressing and reduced the enzymatic activities of superoxide dismutase, polyphenol oxidase, and catalase. The quantitative reverse-transcription polymerase chain reaction analysis showed that the transcript levels of CHS, DFR, F3′5′H, and UFGT genes in the petals of the treatment group continued to increase during the pressing process, and the transcript levels of key genes in the anthocyanin metabolic pathway of the treated samples were higher than those of the control group at the final stage (S4). These results indicated that phenylalanine can effectively diminish the color variation of Petunia petals in the pressing process, which could serve as a theoretical basis for the development of a comprehensive technology system aimed at preserving the color of pressed horticultural plants.

Open Access

Arabidopsis thaliana Flowering locus T (FT) homologs have been shown to be sufficient to trigger flowering and to regulate flowering time in a wide range of plants. However, such a homologue for the perennial ornamental shrub tree peony has not yet been characterized. In this study, we isolated PsFT, which is a closely related FT homolog from reblooming [Paeonia ×lemoinei ‘High Noon’ (HN)] and nonreblooming [P. ×suffruticosa ‘Luo Yang Hong’ (LYH)] cultivars of tree peonies, and identified its potential role in the regulation of flowering time. The PsFT alleles from the two cultivars encode the same protein, which indicates that the polymorphisms observed in the coding region do not contribute to the distinct flowering phenotypes of HN and LYH. Comparative analyses of the PsFT expression patterns in HN and LYH indicated that PsFT might be associated with reblooming. Transgenic A. thaliana plants ectopically expressing PsFT exhibited a phenotype that included significantly early flowering compared with the wild-type (WT) plants. Taken together, our data provide valuable clues for shortening the juvenile periods and extending the flowering periods of perennial woody plants, such as tree peonies.

Free access

By using a modified biotin-streptavidin capturing method, a total of 20 polymorphic microsatellite markers were developed from Moringa oleifera Lam. (Moringaceae), a useful multipurpose tree. Twenty-four domesticated individuals, with germplasms of India and Myanmar, were used to screen polymorphism of these 20 microsatellite markers. The number of alleles per locus ranged from two to six. The expected and observed heterozygosity varied from 0.3608 to 0.7606 and from 0.0000 to 0.8750, respectively. Seven loci were significantly deviated from Hardy-Weinberg equilibrium. The availability of these microsatellite primers would provide a powerful tool for aspects of detailed population genetic studies of M. oleifera.

Free access