Drought has become an important factor limiting crop yields in China. As an important greenhouse horticultural crop in China, the research of tomato (Solanum lycopersicum L. cv. Jinpeng No.10) is of great theoretical and practical significance. In the study, four different relative soil moisture contents (74% to 80%, 55% to 61%, 47% to 52%, and 25% to 30%) were used to induce drought stress. We investigated changes in photosynthetic gas exchange, chlorophyll fluorescence, and other related physiological parameters in response to different relative soil moisture contents. Drought inhibited the photosynthesis of tomato significantly, as shown by a clear decline in the net photosynthetic rate. Our results indicated stomatal limitation and nonstomatal limitation were responsible for the photosynthesis reduction.
Guoting Liang, Junhui Liu, Jingmin Zhang and Jing Guo
Jing Mao, Hongliang Xu, Caixia Guo, Jun Tong, Yanfang Dong, Dongyun Xu, Fazhi Chen and Yuan Zhou
Although tolerance to high temperature is crucial to the summer survival of Iris germanica cultivars in subtropical areas, few physiological studies have been conducted on this topic previously. To remedy this, this study explored the physiological response and expression of heat shock factor in four I. germanica cultivars with varying levels of thermotolerance. The plants’ respective degrees of high-temperature tolerance were evaluated by measuring the ratio and area of withered leaves under stress. Several physiological responses to high temperatures were investigated, including effects on chlorophyll, antioxidant enzymes, proline, and soluble protein content in the leaves of four cultivars. CaCl2 was sprayed on ‘Gold Boy’ and ‘Royal Crusades’ considered being sensitive to high temperatures to study if Ca2+ could improve the tolerance, and LaCl3 was sprayed on ‘Music Box’ and ‘Galamadrid’ with better high-temperature tolerance to test if calcium ion blocker could decrease their tolerance. Heat shock factor genes were partially cloned according to the conserved region sequence, and expression changes to high-temperature stress with CaCl2 or LaCl3 treatments were thoroughly analyzed. Results showed that high temperature is the primary reason for large areas of leaf withering. The ratio and area of withered leaves on ‘Music Box’ and ‘Galamadrid’ were smaller than ‘Gold Boy’ and ‘Royal Crusades’. CaCl2 slowed the degradation of chlorophyll content and increased proline and soluble protein in ‘Gold Boy’ and ‘Royal Crusades’ but had no significant effect on activating peroxidase or superoxide to improve high-temperature tolerance. Genetic expression of heat shock factor in ‘Gold Boy’ and ‘Royal Crusades’ was upregulated by Ca2+ at later stages of leaf damage under high-temperature stress. LaCl3 down-regulated the physiological parameters and expression level of heat shock factor in ‘Music Box’ and ‘Galamadrid’. These results suggest that different I. germanica cultivars have varying high-temperature tolerance and furthermore that Ca2+ regulates their physiological indicators and expression level of heat shock factor under stress.
Jing Tian, Li-Ping Wang, Yan-Juan Yang, Jin Sun and Shi-Rong Guo
Heat tolerance is considered to be an essential feature for cucumber (Cucumis sativus) production, and it has been suggested that higher antioxidant ability could prevent the oxidative damage in plants caused by high-temperature stress. We aimed to investigate whether the application of exogenous spermidine (Spd) increases antioxidant activities and, therefore, elevates the heat tolerance of cucumber. Cucumber seedlings (cv. Jinchun No. 4) showing moderate heat tolerance were grown in climate chambers to investigate the effects of exogenous Spd (1 mm) foliar spray treatment on the activities and isozyme levels of antioxidative enzymes under both high-temperature stress 42/32 °C (day/night) and normal temperature 28/18 °C (day/night). On high-temperature stress, the activities of superoxide dismutase and ascorbate peroxidase were significantly reduced; the catalase activity was initially lower and then increased, whereas the peroxidase activity was initially higher and then decreased. The levels of these isozymes also changed differently. On treatment with exogenous Spd, the activities of these antioxidant enzymes were noticeably enhanced, and the isozyme zymogram expression had some changes. It was concluded that foliar spray with Spd effectively improved the total antioxidant ability of cucumber seedlings and, therefore, enhanced the tolerance of the plants to high-temperature stress.