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  • Author or Editor: Jennifer A. Gargiulo x
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The genus Cryptocoryne (Araceae) contains some of the most commercially important amphibious species used in the aquarium plant trade. However, seed production is rare and vegetative propagation by rhizome division is extremely slow. Procedures for in vitro establishment, axillary shoot proliferation and plantlet acclimatization of Cryptocoryne Becketti Thwaites ex Trimen were determined. Surface sterilized rhizomatous shoot tips were established on a medium consisting of Linsmaier & Skoog mineral salts and organics supplemented with 87.6 mM sucrose, 2.2 μM benzyladenine (BA) and 0.57 μM indole-3-acetic acid (IAA) solidified with 0.8% TC® Agar. Effects of medium supplementation with factorial combinations of BA (0 - 25 μM) and IAA (0 - 10 μM) on axillary shoot proliferation from single node explants were determined after 28 days. Maximum axillary shoot proliferation (`l-fold increase) occurred on medium supplemented with 25 μM BA and 1.0 μM IAA. Excellent microcutting rooting (100%) was achieved by direct sticking in Vergro Klay Mix A. Greenhouse acclimatization of rooted microcuttings was 100%.

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An-assay was developed to determine the level of resistance to the herbicide glyphosate in trangenic seedlings of lettuce. Results of the seedling assay were correlated to results of a similar assay using callus lines of the identical transgenic plants. Transgenic plants were found to be a 32-fold increase in tolerance to glyphosate when compared to wild type plants. This was similar to the response of these transgenic lines in the callus line assay.

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