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Maackia amurensis Rupr. & Maxim. is a leguminous tree with potential for increased use in urban landscapes. Information on the nutrition of M. amurensis is limited. To our knowledge, modulation and N2 fixation have not been reported. Our objective was to examine M. amurensis for nodulation and N2 fixation. Soil samples were collected near legume trees at arboreta throughout the United States, with additional samples from Canada and China. Seedlings were grown for six weeks in a low-N, sterile medium and inoculated with soil samples. Upon harvest, small white nodules were found on the lateral and upper portions of the root systems. Bacteria were isolated from the larger nodules, subculture, and used to inoculate seedlings. Inoculated plants nodulated and fixed N2 as determined by the acetylene reduction assay. We conclude M. amurensis forms N2-fixing symbioses with Rhizobium.
Maackia amurensis Rupr. & Maxim. is a leguminous tree with potential for increased use in urban landscapes. Information on the nutrition of M. amurensis is limited. To our knowledge, modulation and N2 fixation have not been reported. Our objective was to examine M. amurensis for nodulation and N2 fixation. Soil samples were collected near legume trees at arboreta throughout the United States, with additional samples from Canada and China. Seedlings were grown for six weeks in a low-N, sterile medium and inoculated with soil samples. Upon harvest, small white nodules were found on the lateral and upper portions of the root systems. Bacteria were isolated from the larger nodules, subculture, and used to inoculate seedlings. Inoculated plants nodulated and fixed N2 as determined by the acetylene reduction assay. We conclude M. amurensis forms N2-fixing symbioses with Rhizobium.