Search Results

You are looking at 1 - 10 of 20 items for

  • Author or Editor: James R. Steadman x
Clear All Modify Search
Free access

James S. Beaver, James R. Steadman and Dermot P. Coyne

Field reaction of 25 red mottled bean (Phaseolus vulgaris L.) genotypes to common bacterial blight [Xanthomonas campestris pv. phaseoli (Smith) Dye] was evaluated in Puerto Rico over 2 years. The average disease severity (percent leaf area with symptoms) was similar over years. The determinate red mottled genotypes had almost twice as much disease as indeterminate genotypes. Eight of the indeterminate genotypes had significantly less disease than the mean of the field experiments. These genotypes may serve as useful sources of resistance to common bacterial blight. The size of the chlorotic zone around necrotic lesions varied between growing seasons, showing that environment can influence the expression of common bacterial blight symptoms.

Free access

Karen M. Meyer, Greg L. Davis and James R. Steadman

The toxic bait, Adios, was tested with the use of a trap crop in a field experiment at the Univ. of Nebraska during Summer 1998. The insecticide contains the secondary plant metabolites known as cucurbitacins that are highly attractive to the striped and spotted cucumber beetles, Acalymma vittatum and Diabrotica undecimuncata howardi, respectively. These beetles serve as the vector of the bacterial pathogen, Erwinia tracheiphila, which causes severe wilting and eventual death of susceptible cucurbits. The objective of the study was to determine whether treatments of Adios, when applied to a flowering trap crop of resistant squash plants, would lure the cucumber beetles away from the susceptible cucumber plants and reduce bacterial wilt. The study compared the effectiveness of a sprayed trap crop, the direct application of Adios to the cucumber plants and no treatment in a randomized complete-block design. A greater number of beetles were attracted to the sprayed and untreated cucumbers compared to the cucumbers surrounded by the treated trap plants. However, significant numbers of dead beetles were found near the sprayed cucumber plants. Untreated plants showed more feeding damage, diminished fruit quality, and an earlier observation date of wilt symptoms as compared to the other treatments. The treated trap plants and the direct application of Adios were effective in delaying infection in cucumbers compared to the untreated plants in the experimental plots. This treatment may be useful to home gardeners.

Free access

Soon O. Park, Dermot P. Coyne and James R. Steadman

Bean rust, caused by Uromyces appendiculatus, is a major disease of common bean (Phaseolus vulgaris). The objective was to identify RAPD markers linked to the gene (Ur-7) for specific resistance to rust race 59 using bulked segregant analysis in an F2 segregating population from the common bean cross GN1140 (resistant to rust) × Nebraska #1 (susceptible to rust). A single dominant gene controlling specific resistance to race 59 was found in the F2 and was confirmed in the F3. Seven RAPD markers were detected in a coupling-phase linkage with the Ur-7 gene. Coupling-phase RAPD markers OAA11.500, OAD12.550, and OAF17.900 with no recombination to the Ur-7 gene were found. Three RAPD markers were identified in a repulsion-phase linkage with the Ur-7 gene among the three markers at a distance of 8.2 cM. This is the first report on RAPD markers linked to the Ur-7 gene in common bean. The RAPD markers linked to the gene for specific rust resistance of Middle American origin detected here, along with other independent rust resistance genes from other germplasm, could be used to pyramid multiple genes into a bean cultivar for more-durable rust resistance.

Free access

Haytham Z. Zaiter, Dermot P. Coyne and James R. Steadman

Sixteen Alubia lines (15 with long, straight hairs and one with short, hooked hairs on trifoliolate leaves) derived from single-plant selections made in an Alubia landrace (Argentine) were used to evaluate the relation of abaxial leaf pubescence to reaction to rust in a greenhouse experiment. The pinto cultivar UI-114 (short, hooked hairs) was used as a susceptible check. One plant per pot, replicated six times, in a randomized complete-block design was used. The primary leaves and the sixth trifoliolates of all plants from 12- and 50-day-old plants, respectively, were inoculated with a water suspension of urediniospores (105 cells/ml) of rust isolate US-NP85-10-1. Pustule size and rust intensity were assessed 14 days later. No rust pustules were observed on the sixth trifoliolate leaves of the pubescent (long, straight hairs) Alubia lines, but large pustules were observed on the primary leaves (short, hooked hairs) of all Alubia lines and pinto `UI-114'. as well as on the sixth trifoliolate leaf of A-07-2 and pinto `UI-144' (the latter two with short, hooked hairs).

Free access

Soon O. Park, Dermot P. Coyne and James R. Steadman

Bean rust, caused by Uromyces appendiculatus, is an important disease of common bean (Phaseolus vulgaris L.). The objective was to identify RAPD markers linked to the gene (Ur-6) for specific resistance to rust race 51 using bulked segregant analysis in an F2 segregating population from the common bean cross pinto `Olathe' (resistant to rust) × great northern Nebraska #1 selection 27 (susceptible to rust). A single dominant gene controlling specific resistance to race 51 was hypothesized based on F2 segregation, and then was confirmed in the F3 generation. A good fit to a 3:1 ratio for band presence to band absence for each of three markers was observed in 100 F2 plants. Three RAPD markers were detected in a coupling phase linkage with the Ur-6 gene. Coupling-phase RAPD marker OAB14.600 was the most closely linked to the Ur-6 gene at a distance of 3.5 cM among these markers. No RAPD markers were identified in a repulsion phase linkage with the Ur-6 gene. The RAPD markers linked to the gene for specific rust resistance of Middle American origin detected here, along with other independent rust resistance genes from other germplasm, could be utilized to pyramid multiple genes into a bean cultivar for more durable rust resistance.

Free access

Geunhwa Jung, Dermot P. Coyne, James Bokosi, James R. Steadman and James Nienhuis

Dry bean (Phaseolus vulgaris L.) production is limited by bean rust [Uromyces appendiculatus (Pers.) Unger var. appendiculatus]. An effective control strategy for this disease is to breed cultivars with durable resistance. Information on the inheritance, genetic relationships, and mapping of genes with molecular markers for specific resistance (SR), adult plant resistance (APR), and abaxial leaf pubescence (ALP) is needed to pyramid the desired genes for durable resistance. ALP was found to be associated previously with APR in Andean germplasm. The objective here was to identify and map RAPD markers for the genes controlling SR, APR, and ALP and to examine their relationships. Five rust pathotypes were inoculated on the unifoliate leaves of 68 recombinant inbred (RI) lines derived from `PC-50' (presence of SR, APR, and ALP) × XAN-159 (absence of SR, APR, and ALP). SR was determined by a single major gene (Ur-9) to the five rust pathotypes with no detection of recombinants. The fourth trifoliolate leaves were inoculated with one pathotype (A88T1-4b). A single major gene Ur-12 controlled APR to that pathotype. The Ur-9 gene (SR) was independent of and epistatic to the Ur-12 gene (APR). Because of the low number of APR lines in the RI population resulting from the elimination of RI lines with SR, an F2 population was developed from a cross of two homozygous RI lines selected for unifoliate susceptibility to pathotype A88T1-4b and for resistance and susceptibility of the fourth trifoliolate leaves to tag RAPD markers linked to the Ur-12 gene (APR). The single major gene Pu-a determinated ALP and was not linked to Ur-9 (SR) and Ur-12 (ALP). The gene Ur-9 (SR) was linked to RAPD marker J13-1100 at 5 cM and was not assigned to any linkage group or other markers. The gene Pu-a (ALP) was mapped at 20.2 cM from 116.500 and 3.9 cM from marker G3.1150 in linkage group 3. The Ur-12 gene (APR) was mapped at 34.6 cM from marker O13.1350 in linkage group 4b. This is the first report of mapping a gene for APR in common bean.

Free access

Haytham Z. Zaiter, Dermot P. Coyne, James R. Steadman and James S. Beaver

Genetic variation for abaxial leaf pubescence was detected among dry bean (Phaseolus vulgaris L.) cultivars/lines. Inheritance of pubescence (long, straight hairs) was studied in the dry bean crosses of pubescent `Pompadour Checa-50' (Dominican Republic) × eight glabrous cultivars/lines. Segregation for pubescence vs. glabrousness indicated that pubescence was determined by a single major gene or by duplicate recessive epistatic genes, depending on the cross involved. Trichome density (number trichomes per mm) was a quantitative trait. Thus, pubescence was a discrete trait, but trichome density ranged from low to high.

Full access

Dale T. Lindgren, Kent M. Eskridge, James R. Steadman and Daniel M. Schaaf

Severity of rust (Uromyces appendiculatus) and yield of dry edible beans (Phaseolus vulgaris L.) were recorded for 9 years in west-central Nebraska in fungicidal efficacy trials. A weighted analysis of covariance was used to estimate yield loss due to rust. The model fit the data well (R2=0.94), and the slope over all years had a 19 kg.ha−1 decrease in yield for each 1% increase in severity of rust. Yield response within years occurred only through reduction of rust for most fungicide treatments.

Free access

Haytham Z. Zaiter, Dermot P. Coyne, Ralph B. Clark and James R. Steadman

Nine bean cultivars/lines were grown in a Tripp sandy-clay loam (high pH), a Sharpsburg silty clay loam (neutral pH), and a potting mix (equal volume of sand, soil [Sharpsburg silty clay loam], vermiculite and moss pest) (low pH) in greenhouse (one experiment), growth chamber (two experiments), and field (two experiments) in Lincoln, NE, in order to evaluate the leaf reaction of the plants to a Nebraska rust (Uromyces appendiculatus var. appendiculatus) isolate US85-NP-10-1. A factorial arrangement of soil media and cultivars/lines in a randomized complete block design was used in the greenhouse and growth chamber experiments, while a split-plot design (soil media as main plots and cultivars/lines as sub-plots) was used in the field experiments. Significant differences were observed for rust pustule size of cultivars/lines grown on the three different soil media. Plants grown on potting mix medium showed significant Increases in rust pustule size compared with Tripp (high pH) or Sharpsburg silty clay loam soils (neutral pH). A significant interaction occurred between soil media and cultivars/lines for the rust reaction. A positive correlation (R= +0.5) was observed between the increased concentration of C1 and Mn,, and a negative correlation for lower K (R+ -0.44) and soil pH in the potting mix and larger rust pustule size of leaves. These results have implications for plant breeders and pathologists involved in evaluating bean progenies and lines for rust resistance.

Free access

Haytham Z. Zaiter, Dermot P. Coyne, Ralph B. Clark and James R. Steadman

Nine bean cultivars/lines were grown in a Tripp sandy-clay loam (high pH), a Sharpsburg silty clay loam (neutral pH), and a potting mix (equal volume of sand, soil [Sharpsburg silty clay loam], vermiculite and moss pest) (low pH) in greenhouse (one experiment), growth chamber (two experiments), and field (two experiments) in Lincoln, NE, in order to evaluate the leaf reaction of the plants to a Nebraska rust (Uromyces appendiculatus var. appendiculatus) isolate US85-NP-10-1. A factorial arrangement of soil media and cultivars/lines in a randomized complete block design was used in the greenhouse and growth chamber experiments, while a split-plot design (soil media as main plots and cultivars/lines as sub-plots) was used in the field experiments. Significant differences were observed for rust pustule size of cultivars/lines grown on the three different soil media. Plants grown on potting mix medium showed significant Increases in rust pustule size compared with Tripp (high pH) or Sharpsburg silty clay loam soils (neutral pH). A significant interaction occurred between soil media and cultivars/lines for the rust reaction. A positive correlation (R= +0.5) was observed between the increased concentration of C1 and Mn,, and a negative correlation for lower K (R+ -0.44) and soil pH in the potting mix and larger rust pustule size of leaves. These results have implications for plant breeders and pathologists involved in evaluating bean progenies and lines for rust resistance.