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David J. Schuster, James B. Kring, and James F. Price

The sweetpotato whitefly, Bemisia tabaci (Gennadius), was associated with symptoms of a silverleaf disorder of acorn squash (Cucurbita pepo L. cvs. Table King Bush and Table Ace) in cage studies in the greenhouse. Symptoms appeared on uninfested leaves that developed after plants were infested with the whitefly. When the infested lower leaves were removed and the young leaves protected from infestation with insecticides, new growth was asymptomatic or nearly so and symptomatic leaves remained symptomatic. Symptom expression was related more to nymphal density than to adult density since the relationship between log nymph density and symptoms was linear when adult densities were equal.

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Suzanne C. Stapleton, Craig K. Chandler, James F. Price, Daniel E. Legard, and James C. Sumler Jr.

The use of locally grown transplants in Florida strawberry (Fragari×ananassa Duchesne) production has increased since the release of the cultivar Sweet Charlie by the University of Florida in 1992. Previous research has shown that nursery region can influence production patterns of other strawberry cultivars through differences in photoperiod and temperature exposure. Transplants of `Sweet Charlie' strawberry (bareroot and plug plants) from sources representing northern (Canada, Massachusetts, Oregon), southern (Alabama, Florida) and mid latitude (North Carolina) transplant production regions were compared for plant vigor, production, and pest incidence at Dover, Fla. in 1995-96 and 1996-97. Total fruit production was not significantly different forplants among the plant source regions in 1995-96, but total yield from southern source plants in 1996-97 was significantly lower than northern and mid latitude plant sources. Monthly production of marketable fruits varied among the three plant source regions in December, January, and February, during which time market prices fell 46% in 1995-96 and 56% in 1996-97. Plants from northern and mid latitude sources produced significantly greater fruit yield in December than plants from southern sources. Differences among plant sources were detected for early flowering, initial crown size, incidence of foliar disease, arthropod pests, mortality, and fruit weight. Geographic location of strawberry transplant sources influenced fruiting patterns and other components that may affect profitability of `Sweet Charlie' strawberry production in west central Florida.

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Yan Ma, M. Nurul Islam-Faridi, Charles F. Crane, David M. Stelly, H. James Price, and David H. Byrne

To our knowledge, there has been no published technique to produce consistently high-quality slides of somatic chromosomes of roses (Rosa sp.). Therefore, various pretreatments, fixatives, digestions, stains, and maceration and squashing methods were tested to identify a procedure to produce clear, well-spread chromosomes from shoot tips. The best results were obtained after pretreatment in a mixture of 0.1% colchicine and 0.001 m 8-hydroxyquinoline for 4 h, and fixation in 2 acetone: 1 acetic acid (v/v) with 2% (w/v) polyvinylpyrrolidone. The darkest-stained chromosomes were obtained with carbol-fuchsin staining of air-dried cell suspensions that had been spread in 3 ethanol: 1 acetic acid (v/v).