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- Author or Editor: James B. Carstens x
Broccoli flower buds were explanted to a medium consisting of Murashige and Skoog inorganic salts, 30 g sucrose, 100 mg i-inositol, 0.4 mg thiamine·HCl, 80 mg adenine sulfate dihydrate, 170 mg NaH2PO4·H2O, 1 mg indole-3-acetic acid (IAA) and 4 mg 6(γ-, γ-dimethylallylamino)-purine (2iP) and 8 g Bacto-agar per liter. The culture environment was 19–23°C and 1 klx light, 16 hours duration per day from cool white fluorescent tubes. After 5 weeks culture, the explants had produced about 3 shoots per flower bud. The shoots were subcultured on the same medium with a 7.5 fold increase of shoots in 5 weeks. To develop roots, the shoot cuttings were transferred for 2 weeks to a modification of the medium detailed above containing no adenine sulfate dihydrate, NaH2PO4·H2O or 2iP and the IAA was reduced to 0.2 mg per liter. The light intensity was increased to 6 klx. The plantlets were planted in a peat-perlite mix and placed under intermittent mist for 1—3 days. The plants grew in the greenhouse to transplant stage in 6—7 weeks with 96% survival rate.