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- Author or Editor: Jaime E. Lazarte x
Abstract
Single-node cuttings obtained from 2-month-old seedlings of pecan [Carya illinoinensis (Wang.) K. Koch] were induced to break buds and form multiple shoots in liquid, woody plant medium (WPM) and 2% glucose supplemented with 6-benzylamino purine (BA) at 3 mg/liter. In vitro-derived shoots soaked in 1-3 mg/liter indolebutyric acid (IBA) produced adventitious roots in vitro; when soaked for 8 days in 10 mg/liter IBA, they were rooted successfully in soil and acclimated to greenhouse conditions. Etiolation of stock plants did not improve shoot proliferation or rooting under in vitro culture.
Abstract
Treatment of spears of pistillate asparagus (XX) with 5000 mg/liter gibberellic acid (GA3) plus 1000 mg/liter 6-benzyl-amino-9-(tetrahydro-2-pyryl)-purine (PBA) or 2000 and 5000 mg/liter GA3 alone induced development of stamens with sterile anthers. Spears of XY staminate genotype treated with 10 mg/liter PBA or PBA plus 50 g/liter glucose had more hermaphroditic flowers with ovules than untreated flowers; seedless fruits developed after pollination. YY staminate genotype developed pistils with styles following treatment of spears with 100 mg/liter PBA or PBA plus 50 g/liter glucose. Some ovules had well developed integuments and chalaza but no embryo sacs. PBA reduced stamen length and increased anther sterility.