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Response surface methodology was utilized in statistical optimization of three quality factors (the number of multiple shoots, shoot length, and number of leaves) pertaining to regeneration of plantlets from leaf calli of Decalepis hamiltonii Wight. & Arn. (swallow root). The variables evaluated were the levels of sucrose, BA, and NAA each at two different concentrations. Response surfaces for shoot length and multiple shoot number were useful in achieving optimal levels of media constituents and in understanding their interactions, but response surfaces for number of leaves were not. The data indicate that sucrose, BA, and NAA levels may be manipulated to increase or decrease quality factors chosen. This approach may be useful in developing a micropropagation protocol for D. hamiltonii. Chemical names used: benzyladenine (BA); napthaleneacetic acid (NAA).
To remain competitive for federal and state funding, state cooperative extension services must proactively incorporate issues programming and performance-based budgeting. State major program (SMP) design teams, which provide linkages between clientele groups and the research base, must conduct needs assessments to adjust to this new atmosphere of accountability. A case study illustrates how one Florida SMP (FL107, vegetable production, harvest, handling and integrated pest management in Florida) restructured its design team to become more flexible and proactive to target a wider range of outcomes. While still in the implementation phase, this model has already resulted in improved communication within the organization, better addressing extension needs at county level while facilitating reporting at the state level.