K. Haghighi and J.F. Hancock
Restriction fragment analyses of chloroplast DNA (cpDNA) and mitochondrial DNA (mtDNA) were carried out on the principal cytoplasms of northern highbush cultivars and one representative of Vaccinium ashei Reade. Twenty-three restriction enzymes were used to identify variation and clarify mode of organelle inheritance. All species and genotypes displayed identical cpDNA fragment patterns, but high degrees of polymorphism were observed in the mitochondrial genomes. `Bluecrop' and `Jersey' did not appear to have `Rubel' cytoplasm as was previously believed. All hybrids contained maternal-type mtDNA.
S. Serce and J.F. Hancock
A common complaint with day-neutral strawberries is that they perform poorly in mid-summer heat. Since most modern day-neutral cultivars are derived from the same Fragaria virginiana ssp. glauca clone from Utah, we felt it prudent to search for alternate sources of day-neutrality that were more heat-tolerant. We compared the sexual and vegetative performance of nine F. virginiana clones from a wide range of environments including the Utah site, and four F. × ananassa day-neutral types (`Aromas', `Fort Laramie', `Ogallala', and `Tribute') under constant temperatures of 18, 22, 26, and 30 °C and 12-h days. `Aromas' and `Tribute' carry the Utah source of day-neutrality, while `Fort Laramie' and `Ogallala' are old cultivars that have a different, complex background. After a 4-week period of acclimation, we counted the number of crowns, inflorescences, flowers, stolons, and daughter plants that emerged over a 10-week period, and measured the dry weights of component parts. ANOVA tables revealed that temperature regime (T), genotypes (G), and T*G were significant for flower number (FLN) and total dry matter accumulation, while species and T*G were significant for daughter plant number (DPN). Mean FLNs across the four temperatures were 6.8, 3.7, 3.3, and 1.2, while mean DPNs were 0.7, 0.9, 0.7, and 1.8. F. virginiana clones averaged 3.8 FLNs and 1.8 DPNs, while the F. × ananassa clones averaged 4.1 FLNs and 0.2 DPNs. There was generally more variability among the F. virginiana clones than the F. × ananassa clones, but the F. × ananassa cultivars, `Fort Laramie' and `Ogallala', performed best at 30 °C. The Wasatch clone did not flower in any treatment, suggesting it is not day-neutral.
S. Serçe and J.F. Hancock
Luping Qu and J.F. Hancock
RAPD markers were used to determine the level of heterozygosity transmitted via 2n gametes from V. darrowi cv. Florida 4b (Fla 4B) to interspecific hybrids with tetraploid V. corymbosum cv. Bluecrop. The tetraploid hybrid US 75 was found to contain 70.6% of Fla 4B's heterozygosity, a value consistent with a first division restitution (FDR) mode of 2n gamete production. Crossovers during 2n gamete formation were evidenced by the absence of 16 dominant alleles of Fla 4B in US 75, and direct tests of segregation in a diploid population involving Fla 4B. RAPD markers that were present in both Fla 4B and US 75 were used to determine the mode of inheritance in a segregating population of US 75 × V. corymbosum cv. Bluetta. More than 30 homozygous pairs of alleles were located that segregated in a 5:1 ratio, indicating US 75 undergoes tetrasomic inheritance.
J.D. Carlson and J.F. Hancock Jr.
Fifteen years of Michigan harvest data for highbush blueberry (Vacciniun corymbosum L.) were used in conjunction with daily maximum and minimum temperatures to determine appropriate heat-unit models for first-picking dates of 13 cultivars. For each cultivar, an optimal heat-unit model was chosen after evaluating the performance of a standard method with 72 combinations of three variables: a) starting date for the heat-unit accumulations (SDATE), b) low-temperature threshold (TLOW), and c) high-temperature threshold (THIGH). The optimal model sought to include the most important criteria values with respect to model performance and to minimize the average square of the prediction error (days) and the range in that error. Compared with a strict calendar-day method of estimating harvest dates, the heat-unit models reduced the standard deviation of the prediction error from 22% to 69%, depending on cultivar.
M. Sakin, J.F. Hancock and J.J. Luby
The genes that determine cyclic flowering in all commercially grown cultivars of strawberry (Fragaria ×ananassa Duch.) were derived from a single source of F. virginiana ssp. glauca from the Wasatch Mountains in Utah. To broaden the germplasm base of cyclic flowering cultivars, we evaluated the reproductive characteristics of 5 to 10 colonies of F. virginiana ssp. glauca from each of 32 Rocky Mountain sites ranging in elevation from 700 to 2900 m. Populations at high and low elevations had high percentages of putative day neutrals with cyclic flowering (43% to 100%) and hermaphrodites (20% to 80%), although most hermaphrodites were only partially fertile. There was also little association between elevation and crown numbers or flower number per cycle, but the total number of flowers per plant was negatively correlated with elevation. Fruit size was not significantly correlated with fruit number. When the data were subjected to a principal component analysis, two distinct groups were identified: one from the Black Hills of South Dakota and the other from low-elevation sites in Idaho and northwestern Montana. These patterns mirrored previously described patterns based on leaf traits.
J.D. Caldwell, J.F. Hancock and J.A. Flore
The commercial strawberry Frageria × ananassa and several clones of F. chiloensis and F. virginiana were preconditioned in growth chambers under the following conditions: 500 μmol m-7s-1 PAR and 20° day, 10° night or 30° day and 20° night. After at least 3 weeks of preconditioning at the two different temperature regimes, leaf photosynthetic rate (A) was determined for temperatures ranging from 10 to 35° in 2-3° increments with an open gas exchange system under laboratory conditions. The objective was to determine if F. virginiana and F. × ananassa can photosynthesize at higher temperatures than F. chiloensis, and if any of these would acclimate to higher temperatures. F. chiloensis did not acclimate to higher temperatures, and bad maximum A between 16 and 20°. F. virginiana did acclimate to higher temperatures, with maximum A for the low temp treatment between 18 and 24°, and for the high temp treatment between 24 and 30°. The commercial cultivars of `Earliglow' and `Redchief' acclimated to higher temperature and responded similar to F. virginiana.
Christopher L. Owens, J.F. Hancock and A.F. Iezzoni
Sour cherry and strawberry are examples of two Rosaceous species that often suffer crop reductions due to spring freezes. Breeding for improved floral freezing tolerance has the potential to mitigate the susceptibility of these plants to spring frosts. In model plant systems, researchers have been able to identify genes that play a role in freezing tolerance by initially searching for mRNAs regulated in response to cold temperatures. To search for cold-responsive freezing-tolerance genes in strawberry and sour cherry, it is necessary to first define their cold acclimation response. To test the hypothesis that sour cherry and strawberry flowers have the ability to cold acclimate, blooming plants were exposed to 4 °C and 16 h light for 14 days. Sour cherry styles and strawberry receptacles from open, fully developed flowers were excised, and electrolyte leakage curves were generated over a range of subzero temperatures. The temperature at which 50% electrolyte leakage (EL50) occurred was used to compare treatments. The flowers of two strawberry cultivars were tested for the ability to cold acclimate. Non-acclimated `Chandler' receptacles had an EL50 of -2.9 °C, while non-acclimated `Honeoye' had an EL50 of -3.4 °C. Conversely, acclimated `Chandler' receptacles had an EL50 of -7.7 and acclimated `Honeoye' receptacles had an EL50 of -8.7 °C, both are significantly different from non-acclimated values (P ≤ 0.01). Additionally, sour cherry styles were collected from the field at full bloom from a mapping population of 86 individuals from the cross `Rheinische Schattenmorelle' × `Erdi Botermo' and acclimated as previously described. The EL50 of the 86 progeny ranged from approximately -2.0 to -6.0 °C.