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- Author or Editor: J.D. Williamson x
Several planting treatments modified vegetative and reproductive growth of young, own-rooted peach (Prums persica) trees evaluated at two levels of irrigation in a high-density orchard (5000 trees/ha). Trees planted in auger holes, narrow herbicide strips, and in fabric-lined trenches, but not those from raised beds, were smaller than control trees set in holes dug with a shovel. After two growing seasons, trees planted in the fabric-lined trenches were smaller and had more flowers per node and greater flower bud densities than trees in other planting treatments. Yield efficiency was greatest for this treatment, although fruit size was small throughout the orchard. Irrigation rates did not affect fruit yield or size. The effects of irrigation rate on vegetative growth were small compared to differences among planting treatments.
Abstract
The cover depicts a cross-sectional photomicrograph of a root from peach treated with a soil application of paclobutrazol. The 14-μm section was taken about 2 mm from the root apex. The tissue was fixed and embedded by conventional methods similar to those described by Sass (8), then doublestained with safranin and fast green. Photomicrographs were taken at × 180 with Ektachrome film (Kodak) using a Leitz Dialux 20 research microscope equipped with an Orthomat automatic microscope camera.
Planting treatments were evaluated for their influence on shoot development and root distribution of own-rooted `Redhaven' peach [Prunus persica (L.) Batsch] trees planted to high density (5000 trees/ha). Planting in fabric-lined trenches (FLT) or narrow herbicide strips (NHS) reduced the diameter and length of primary shoots, the number and combined length of second-order shoots, and the total length of shoots. Flower density, the number of flowers per node, and the percentage of nodes containing one or more flowers were increased for FLT trees but not for NHS trees when compared with controls. The length of primary shoots increased quadratically for all treatments with increasing limb cross-sectional area (LCA). The total length of shoots increased more with increasing LCA for controls than for FLT trees. The number of flowers per shoot increased linearly for all treatments with increasing LCA values. Root concentration decreased with increasing soil depth and distance from tree rows for all treatments. Reduced widths of weed-free herbicide strips had little effect on root distribution. Roots of FLT trees were reduced in number and restricted vertically and laterally when compared with other planting treatments. The FLT treatment modified shoot development by reducing the length of total shoots and length of primary shoots across LCA values measured when compared with NHS and control-treatments.
Abstract
Seed of 11 cultivars of snap bean, Phaseolus vulgaris L., were separated by aerodynamic properties in a vertical air column. Seed physical characteristics associated with air column separation were weight, density, volume, diameter, and length. The separation technique did not affect seed germination, seedling emergence, or plant survival at full expansion of the first trifoliate leaf growth stage. However, seed remaining in the air column after aspiration produced fewer weak plants and fewer plants with root rot at the first trifoliate leaf. These seed produced a greater plant stand, a greater pod weight per plant, a more uniform pod size distribution, and a greater yield at harvest than the seed removed. Yield from seed remaining after air column aspiration was 21% greater than from non-graded seed.
The effects of hydrogen cyanamide (H2CN2) sprays on vegetative and reproductive bud growth and development were evaluated for `Climax' rabbiteye (Vaccinium ashei Reade) and `Misty' southern highbush blueberry (V. corymbosum L. hybrid). `Climax' plants were sprayed with 0% or 1% H2CN2 (v/v) at each of several time intervals or flower bud growth stages following either 270 or 600 hours of artificial chilling. `Misty' plants were sprayed with 0%, 1%, or 2% H2CN2 (v/v) immediately after exposure to 0, 150, or 300 hours of artificial chilling. H2CN2 application to `Climax' plants at 3 days after forcing (DAF) and at 10% to 30% stage 3 flower bud development dramatically accelerated leafing, and only minimal flower bud damage was observed at these application times. For `Misty', vegetative budbreak was increased and advanced by both H2CN2 spray concentrations, regardless of pretreatment chilling levels; the number of vegetative budbreaks per plant increased with increased concentration. Timing of anthesis did not appear to be affected by H2CN2, but fruit maturity was hastened. Increased pretreatment chilling also hastened fruit development. This effect on maturity appears to be due primarily to increased and accelerated vegetative budbreak, which probably increased leaf: fruit ratios. Greater flower bud mortality from H2CN2 occurred in nonchilled plants than in those chilled for 150 or 300 hours, especially at 2% H2CN2. These results indicate that H2CN2 has potential value in stimulating vegetative bud development, which potentially hastens maturity in blueberries grown under the mild winter conditions of the Southeast. However, spray concentration and timing of application will be critical to successful use of this compound.
Abstract
Paclobutrazol applied at 37 mg per plant to container-grown, own-rooted peach [Prunus persica (L.) Batsch ‘Redhaven’] trees reduced vegetative growth (compared with no treatment) as measured by shoot extension, leaf size, and shoot dry weight. All paclobutrazol treatments (foliar and/or soil) increased root tip diameter and reduced unsuberized root length. Paclobutrazol increased the size of the cortex parenchyma cells and resulted in radial rather than longitudinal elongation of the innermost layer of cortex cells. These changes in cell shape and size were primarily responsible for increasing root tip diameter. Chemical name used: (R*,R*)-(±)-β-[(4-chlorophenyl)methy]-α-(l,l-dimethylethyl)-1H-1,2,4-triazole-l-ethanol (paclobutrazol).
Abstract
Ethylene is produced by cucumber fruits (Cucumis sativus L.), at a rate which is size dependent. Small fruits (<2.6 cm diam) produced substantially more ethylene/kg fruit than did large fruits (2.6-3.8 and 3.8-5.1 cm diameter). Respiration was similarly affected. Mechanically harvested fruits produced 2 to 3 times more ethylene than did hand-harvested fruits. Texture profile analysis (TPA) of cross-sections of fruits treated 48 hr with 0, 0.1, 0.5, 1.0, 5.0 and 10.0 µl/liter ethylene indicated little change in textural parameters at concentrations below 10.0 µl/liter. Ethylene treatment, especially high concentrations, decreased fruit chlorophyll content. Greatest chlorophyll loss was at the stem-end of the fruit. Ambient concentrations of ethylene in well-ventilated trucks of cucumbers were not great enough to present a quality problem for processing cucumbers.
Increasing salinity of agricultural soils may ultimately limit the sustainability of food production in some areas of the world. Work from our laboratory and the labs of others demonstrates that mannitol, a six-carbon sugar alcohol, is important as a stress-related metabolite in some plants. Mannitol helps plants resist the damaging effects of stressful growth environments, such as drought, high soil salinity, and perhaps attack by microorganisms that cause plant diseases. In the long run, we hope to genetically engineer plants to produce and use mannitol for increased productivity and tolerance to environmental stresses. Basic information about how plants regulate those genes important to mannitol metabolism is of critical importance to this long-term goal. Our laboratory discovered an enzyme, mannitol dehydrogenase, that is the first critical biochemical step in mannitol use in vascular plants. Later, we cloned the gene for this enzyme. We discovered that hexose sugars “turn off” the expression of this gene. So, as long as adequate sugars are available for energy, maintenance, and growth, the production of the mannitolusing enzyme is repressed. After the sugars are gone, mannitol dehydrogenase is produced very rapidly, and this allows mannitol to be used metabolically. This type of gene regulation is ideally designed to help plants cells conserve mannitol as long as possible, which in turn allows the cells to retain stress tolerance as long as possible.
Mannitol, a six carbon sugar alcohol, is widely distributed in nature and is a major phloem-translocated photoassimilate in celery. II may also function as a compatible osmolyte providing stress tolerance. Until recently, little was known about the route of mannitol catabolism in sink tissues of higher plants. An enzyme. mannitol dehydrogenase. (MDH) that oxidizes mannitol to mannose utilizing NAD as the electron acceptor was discovered (Arch. Biochem. Biophys. 1991. 298:612-619) in “sink” tissues of celery and celeriac plants. The activity of the enzyme is inversely related to tissue mannitol concentration in various parts of celery plants suggesting a role for the enzyme in mannitol catabolism. In osmostressed celery plants, the activity of the enzyme in sink tissues decreases as mannitol accumulates.
Celery cells growing heterotrophically in suspension culture utilize either sucrose or mannitol as the sole carbon source and grow equally well on either carbohydrate. Mannitol-grown cells contain more MDI-I activity than sucrose-grown cells, and the activity of the enzyme is correlated with the rate of depletion of mannitol from the culture medium. Cells growing on mannitol contain an internal pool of mannitol but little sugar. Cells growing on sucrose contain internal sugar pools but no mannitol. Mannitol-grown cells are also more salt tolerant than cells grown on sucrose. Our laboratory is involved in studies of the physiological role of the mannitol oxidizing enzyme in regulating mannitol utilization and the role of the enzyme in regulating mannitol pool size during salt and osmostress in both celery plants and celery suspension cultures. Current studies on the molecular control of expression of the enzyme will be discussed.