In vitro propagated shoots of the apple rootstock, Antonovka 313 (Malus pumila Mill.)9 were rooted successfully in vitro. Roots became visible in 6-8 days, and 100% rooting after 2 weeks was achieved consistently in shoots cultured on modified Murashige and Skoog (MS) salt medium supplemented with 0.25 μm indole-3-butyric acid (IBA). Sucrose was the most influential medium component for rhizogenesis. Inorganic nutrients, IBA and vitamins did not influence rooting. Omitting activated charcoal caused only a slight decrease. A 1.5% sucrose solution added to a peat-vermiculite growing medium in vitro resulted in higher rooting than in treatments without sucrose.