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North American Vitis species and hybrids thereof have been the source of rootstocks for V. vinifera for the last century. Collection and evaluation of native Vitis in north-central Texas, western Texas, and New Mexico have been made to determine their current status. Known geographical pockets of grapevines were visited, with specimens taken and identified by comparison to herbarium collections and published descriptions. In locals where more than one species existed, many natural hybrids with varying morphological characteristics have become established. In North Central Texas, two areas were visited. The first was Tarrant, Parker and Wise counties where three grapevine species (V. mustangensis, V. cinerea var. helleri, and V. vulpina) and many hybrids were observed. The second was Wilbarger County where V. acerifolia was found growing in the south while V. ×doaniana was found growing in the north. West Texas was primarily populated with V. acerifolia with the exception of the Silver Falls Canyon area in Crosby County where hybrids of V. acerifolia, V. arizonica and V. riparia were observed. In New Mexico, two areas were visited: San Miquel County (North Central region), where V. acerifolia, V. arizonica, and V. riparia were observed and Eddy County (southern New Mexico) where V. arizonica was observed. A rich diversity of Vitis germplasm appears to remain in these habitats.