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  • Author or Editor: J. Heursel x
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The short-term effects of photosynthetic photon flux (PPF), day/night temperatures and CO2 concentration on CO2 exchange were determined for two Phalaenopsis hybrids. At 20 °C, the saturating PPF for photosynthesis was 180 μmol·m-2s-1. At this PPF and ambient CO2 level (380 μL·L-1), a day/night temperature of 20/15 °C resulted in the largest daily CO2 uptake. Higher night temperatures probably increased the respiration rate and lowered daily CO2 uptake in comparison with 20/15 °C. An increase in the CO2 concentration from 380 to 950 μL·L-1 increased daily CO2 uptake by 82%.

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Abstract

The chromosome number of 47 cultivars of Rhododendron simsii Planch, were all diploid (2n = 26) except ‘Euratom’ which was triploid (3n = 39). A number of R. simsii cultivars from China and Thailand, as well as R. indicum (L.) Sweet., R. tamurae (Makino) Masam., R. keiskei Miq., and R. kiusianum Makino were found to be diploid.

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Ploidy level was determined for six species and 88 cultivars of the Rhododendron subgenus Tsutsusi. High-resolution flow cytometry of nuclear DNA was performed on macerated plant tissue. All plants analyzed were diploid (2n = 26) with the exception of `Euratom', `Euratom Orange', and `Red Wing', which were triploid (3n = 39), and `Casablanca Tetra', which was found to be a cytochimera: mixoploid (2n + 4n) in the LI and LII, but tetraploid in the LIII. The described method has proven to be useful in screening a large population of rhododendrons. Analysis of different organs and plant tissues was easily accomplished through flow cytometry, and has proven useful in determining the ploidy of different histogenic layers.

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