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  • Author or Editor: Ivana Gribaudo x
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Leaf fragments of Ficus lyrata Warb. were excised, surface-sterilized, and inoculated on a modified Nitsch and Nitsch medium (thiamine, 15 μm) supplemented with 24 μm BA + 24 μm 2iP + 24 μm kinetin + 12.5 μm IBA. After 3 months, the explants started to proliferate on the edge of the cuttings, producing many adventitious buds that developed into shootlets. During a further growth period of 1 month, full elongation was attained. The plants were then transferred to a rooting medium containing Knop’s macroelements, modified Nitsch & Nitsch’ microelements, amino acids and vitamins, and 5 μm of NAA. After 1 week on this medium, the shootlets were deprived of NAA and thus induced to root within 1 week. Chemical names used N-(phenylmethyl)-1H-purin-6-amine (BA); N-(3-methyl-2-butenyl)-1H-purin-6-amine (2iP); N-(2-furanylmethyl)-1H-purin-6-amine (kinetin); 1H-indole-3-butanoic acid (IBA); 1H-indole-3-acetic acid (IAA); 1-naphthaleneacetic acid (NAA).

Open Access

Plantlets of Vitis vinifera L. `Moscato Bianco' were grown in vitro in cellulose plugs (Sorbarods) saturated with a modified Murashige and Skoog rooting medium. Both the inclusion of 0.5-l mg paclobutrazol/liter in the rooting medium and the use of culture vessels that reduce the relative humidity from 100% to 94% improved resistance of plantlets to wilting after transplanting. Maximum benefit was obtained with a combination of paclobutrazol and reduced humidity; it resulted in smaller stomatal apertures, shorter stems, reduced leaf area, and more and thicker roots. Chemical names used: (2RS, 3RS)-1-(4-chlorophenyl)-4,4-dimethyl-2-(1H-1,2,4-triazol-1-yl) pentan-3-01 (paclobutrazol).

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