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  • Author or Editor: Irena Sińska x
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Evolution of endogenous ethylene (C2H4) increased in seed of apple (Malus domestica Borkh.) in a stepwise manner during 40 days of stratification. C2H4 evolution during culture of isolated embryos depended on the stratification stage. Cultured embryos of seeds stratified 0 to 30 days increased in C2H4 production immediately preceding germination, but embryos stratified 40 days showed a simultaneous rise in C2H4 production with germination. During embryo culture, C2H4 was produced by both embryonic axes and cotyledons. Application of (2-chloroethyl)phosphonic acid (ethephon) to the seed-stratification medium increased embryo germination. Trapping endogenously produced C2H4 with Hg(CIO4)2 during stratification did not affect embryo germination, but trapping it during culture of isolated embryos completely inhibited germination.

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