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  • Author or Editor: Irena Sińska x
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Abstract

Evolution of endogenous ethylene (C2H4) increased in seed of apple (Malus domestica Borkh.) in a stepwise manner during 40 days of stratification. C2H4 evolution during culture of isolated embryos depended on the stratification stage. Cultured embryos of seeds stratified 0 to 30 days increased in C2H4 production immediately preceding germination, but embryos stratified 40 days showed a simultaneous rise in C2H4 production with germination. During embryo culture, C2H4 was produced by both embryonic axes and cotyledons. Application of (2-chloroethyl)phosphonic acid (ethephon) to the seed-stratification medium increased embryo germination. Trapping endogenously produced C2H4 with Hg(CIO4)2 during stratification did not affect embryo germination, but trapping it during culture of isolated embryos completely inhibited germination.

Open Access