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Abstract
In the article “Gelling Agent and Growth Regulator Effects on Shoot Vitrification of ‘Gala’ Apple in Vitro”, by P.-L. Pasqualetto, R.H. Zimmerman, and I. Fordham (J. Amer. Soc. Hort. Sci. 111:976–980, November 1986), two corrections should be noted: 1) On p. 979, the 11th line in the discussion, Fig. 1 should read Fig. 2. 2) The correct version of Table 1 is printed below.
Abstract
Shoot tips of ‘Gala’ apple (Malus domestica Borkh.) were grown on proliferation medium containing Murashige and Skoog salts, supplemented with 0.56 mm myo-inositol; 1.2 μm thiamine-HCl; 2.2 or 4.4 μm BA; 0.005, 0.05, or 0.5 μm IBA; and 1.3 μm GA3. The media were solidified with Gelrite at concentrations from 0.5 to 1.5 g·liter−1 plus agar (Sigma) from 0.5 to 4.0 g·liter−1. Vitrification was influenced both by gelling agent and BA concentration, whereas the IBA concentration had little effect. Increasing either agar or Gelrite concentration resulted in a decreasing percentage of vitrified explants. The higher BA level generally increased vitrification, but increasing the agar concentration tended to reduce the difference in effect of the 2 BA levels. Certain combinations of gelling agents produced no vitrification with no reduction in shoot proliferation, and with a clarity of the medium approaching that of Gelrite alone. Chemical names used: N-(phenylmethyl)-lH-purin-6-amine [benzyladenine (BA)]; lH-indole-3-butanoic acid (IBA); and gibberellic acid (GA3).