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Open access

Xiongwen Chen and Hua Chen

Chinese Torreya (Torreya grandis cv. Merrillii) is an important economic tree in China, but there are limited studies on its seed production. We analyzed the patterns of historical seed production at two major sites (Zhaojiazhen and Jidongzhen) for Chinese Torreya from different perspectives. The results indicated that there were no 3-year or multiyear cycles in its seed production. A positive correlation existed between the average seed production and the average annual air temperature in 5 or 10 years at both study sites. There was no trend of the increasing coefficient of variance (cv) in seed production, but the cv generally increased before 1975, and became flat after that time. Frequency power law existed in seed production at both sites, but Taylor’s Law existed only at Zhaojiazhen. The multiscale entropy decreased with time scales, and the patterns were similar at both sites. Our research results provide a new understanding of seed production for Chinese Torreya.

Open access

Xia Chun-hua and Chen Jian-hua

Anthurium andraeanum Lind. is an economically important potted and fresh-cut flower species. However, reduced anthocyanin content under shaded cultivation impacts the color of the spathe, which has negative implications for the marketability of A. andraeanum. Thus, the present study evaluates the use of oyster shell supplementation to the cultivation medium for improving anthocyanin content. Appropriate calcium (Ca) can improve the activity of phenylalanine ammonia lyase (PAL), and PAL activity is positively correlated with anthocyanin content; nitrogen (N) and phosphorus (P) nutrients are closely related to anthocyanin synthesis. N and P nutrients and Ca can alleviate the color symptoms of A. andraeanum when anthocyanin content decreases under weak light (under 220 μmol·m−2·s−1). Microdissolution of calcium carbonate, the main component of oyster shell, can provide better exogenous Ca and adsorb slow-release N and P. Selecting appropriate oyster shell fragments will be the key to A. andraeanum experimental cultivation under low light conditions. Using regression models and response surface methodology (RSM), the relationships between oyster shell fragments and anthocyanin content are promulgated. The main findings indicated that the Ca released from 286-mg oyster shell fragments at pH 5.5 significantly increased the activity of PAL in the pedicel under weak light within 8 hours. At pH 5.9, 375-mg oyster shell fragments could significantly adsorb N and P nutrients within 4 to 14 hours. In conclusion, 286 to 375 mg oyster shell fragments at pH 5.5 to 5.9 could stabilize slow-release fertilizer source and significantly increase anthocyanin content in A. andraeanum spathe.

Free access

Zhiyi Bao, Bo Chen and Hua Zhang

Loropetalum chinense var. rubrum accessions in China have not been adequately characterized for their morphological traits. Such characterization would be helpful in the development of improved cultivars and in cultivar classification. In this study, the morphological traits of 23 accessions were evaluated in spring, summer, and autumn to determine their phenotypic diversity. Cluster analysis with average distance was performed for the main traits of leaf and flower using data processing system software. The morphological investigation indicated that the number of flowering accessions and the flower number of L. chinense var. rubrum in spring were more than those in autumn. Only one accession (no. 13) yielded several flowers in summer. Some accessions had the same or similar color of leaf or flower in spring and autumn. Two accessions had the same flower color in spring and autumn, whereas others showed slightly different colors in spring and autumn. The 23 entries were grouped into four clusters in spring and five clusters in autumn based on multivariate analysis of nine classification variables. Each cluster had some specific characteristics of its own. Generally, the cluster formed first because of the similarity in leaf color. The accessions with similar flower color formed subclusters within a cluster. These accessions are an important resource for the establishment of a core collection of L. chinense var. rubrum in the world. Several accessions with good qualities were selected and should be further tested for horticultural merit.

Free access

Ying Chen, Xinlu Chen, Fei Hu, Hua Yang, Li Yue, Robert N. Trigiano and Zong-Ming (Max) Cheng

Agave species are economically important plants in tropical and subtropical desert ecosystems as ornamentals as well as potential bioenergy crops. However, their relatively long life cycles and the current lack of biotechnology tools hinder their breeding. In this study, an efficient system for micropropagation was developed for Agave americana L. by using basal stems as explants and grown on a modified Murashige and Skoog medium (MSI) or a 1/2 MSI medium supplemented with various concentrations of 6-benzylaminopurine (BA) for shoot proliferation. The highest number of shoots (18.5 shoots/explant) from basal stems was obtained on MSI supplemented with 13.32 μM BA. An efficient shoot regeneration system was also developed from leaf tissues. Combinations of auxin with cytokinin, basal media, and leaf regions were optimized for shoot induction. Adventitious shoot formation from leaf segments was induced and proliferated with combination ranging of 0.54 to 2.68 μM [α-naphthaleneacetic acid (NAA)] with 8.88 to 13.32 μM (BA), and the maximum frequency (≈69%) was obtained with 2.68 μM NAA plus 13.32 μM BA. MSI medium and the basal segment of leaf affected shoot induction. The highest rooting frequency and mean number of shoots occurred in 1/2 MSI containing with 4.92 μM indole-3-butyric acid (IBA) alone (90%, 3.4) or 1.48 μM IBA plus 1.61 μM NAA (92%, 5.2). Survival of in vitro plantlets after transfer and acclimatization to ex vitro conditions was 87%. This is the first complete protocol for micropropagation of A. americana.

Free access

Fure-Chyi Chen, Wan-Ling Wu, Chun-Hua Pan, Irwin Yau-Yuan Chu and Yau-Wen Yang

Molecular markers by random amplified polymorphic DNAs were used to evaluate the genetic variation among different Oncidium accessions. It is possible to distinguish different registered Oncidium hybrids, including Gower Ramsey, Sweet Sugar, and Taka using nine random primers. Furthermore, variation was also detected within different cultivars derived from same hybrids. For example, several cultivars of Gower Ramsey could be distinguished based on molecular markers. Based on dendrogram, the investigated cultivars were clustered into several groups. Onc. Gower Ramsey and its selected cultivars were in one group. Onc. Sweet Sugar, Onc. Taka and Onc. Sharry Baby `Sweet Fragrance' were clustered in separate groups.

Full access

Li-Hong Gao, Mei Qu, Hua-Zhong Ren, Xiao-Lei Sui, Qing-Yun Chen and Zhen-Xian Zhang

Single-slope, energy-efficient solar greenhouses in China use solar energy as the sole source of light and heat for winter crop production in the region between latitudes 32°N and 43°N. The use of solar greenhouses has greatly reduced energy demand and carbon dioxide (CO2) emissions. Solar greenhouses are the best structure for growing winter horticultural crops in China, and have been adopted by countries such as Japan, Korea, and Russia. Increased proliferation of efficient solar greenhouses in China may contribute to solving worldwide problems such as the energy crisis and global climate change. This article summarizes the structure, function, application, and ecological benefits of energy-efficient, single-slope solar greenhouses in China, based on 20 years of systematic studies. We hope this technology can be applied to regions of similar climate to help reduce energy consumption and CO2 emissions.

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Canbin Chen, Pengyang Wu, Fangfang Xie, Luyang Sun, Yemiao Xing, Qingzhu Hua, Zhike Zhang, Jianye Chen, Jietang Zhao, Guibing Hu, Yonghua Qin, Jiaqiang Li and Yaoxiong Ye

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Chen Chen, Meng-Ke Zhang, Kang-Di Hu, Ke-Ke Sun, Yan-Hong Li, Lan-Ying Hu, Xiao-Yan Chen, Ying Yang, Feng Yang, Jun Tang, He-Ping Liu and Hua Zhang

Aspergillus niger is a common pathogenic fungus causing postharvest rot of fruit and vegetable, whereas the knowledge on virulence factors is very limited. Superoxide dismutase [SOD (EC 1.15.1.10)] is an important metal enzyme in fungal defense against oxidative damage. Thus, we try to study whether Cu/Zn-SOD is a virulence factor in A. niger. Cu/Zn-SOD encoding gene sodC was deleted in A. niger [MA70.15 (wild type)] by homologous recombination. The deletion of sodC led to decreased SOD activity in A. niger, suggesting that sodC did contribute to full enzyme activity. ΔsodC strain showed normal mycelia growth and sporulation compared with wild type. However, sodC deletion markedly increased the cell’s sensitivity to intracellular superoxide anion generator menadione. Besides, spore germination under menadione and H2O2 stresses were significantly retarded in ΔsodC mutant compared with wild type. Further results showed that sodC deletion induced higher superoxide anion production and higher content of H2O2 and malondialdehyde (MDA) compared with wild type, supporting the role of SOD in metabolism of reactive oxygen species (ROS). Furthermore, ΔsodC mutant had a reduced virulence on chinese white pear (Pyrus bretschneideri) as lesion development by ΔsodC was significantly less than wild type. The determination of superoxide anion, H2O2, and MDA in A. niger-infected pear showed that chinese white pear infected with ΔsodC accumulated less superoxide anion, H2O2, and MDA compared with that of wild type A. niger, implying that ΔsodC induced an attenuated response in chinese white pear during fruit–pathogen interaction. Our results indicate that sodC gene contributes to the full virulence of A. niger during infection on fruit. Aspergillus niger is one of the most common species found in fungal communities. It is an important fermentation industrial strain and is also known to cause the most severe symptoms in fruit during long-term storage (Pel et al., 2007). Meanwhile, plants activate their signaling pathways to trigger defense responses to limit pathogen expansion. One of the earliest host responses after pathogen attack is oxidative burst, during which large quantities of ROS are generated by different host enzyme systems, such as glucose oxidase (Govrin and Levine, 2000). ROS such as singlet oxygen, superoxide anion, hydroxyl (OH), and H2O2 are released to hinder the advance of pathogens (Gara et al., 2003). ROS can react with and damage cellular molecules, such as DNA, protein, and lipids, which will limit fungal propagation in the host plant (Apel and Hirt, 2004).

Full access

Ting-Ting Li, Zhi-Rong Li, Kang-Di Hu, Lan-Ying Hu, Xiao-Yan Chen, Yan-Hong Li, Ying Yang, Feng Yang and Hua Zhang

Kiwifruit (Actinidia deliciosa) is a typical climacteric fruit, and its ripening is closely associated with ethylene. In this study, we present evidence that H2S alleviated ethylene-induced ripening and senescence of kiwifruit. Kiwifruit were fumigated with ethylene released from 0.4 g·L−1 ethephon solution or H2S with 1 mm sodium hydrosulfide (NaHS) as the donor or in combination. Fumigation with ethylene was found to accelerate kiwifruit ripening and H2S treatment effectively alleviated ethylene-induced fruit softening in parallel with attenuated activity of polygalacturonase (PG) and amylase. Ethylene + H2S treatment also maintained higher levels of ascorbic acid, titratable acid, starch, soluble protein, and reducing sugar compared with ethylene group, whereas suppressed the increase in chlorophyll and carotenoid. Kiwifruit ripening and senescence under ethylene treatment was accompanied by elevation in reactive oxygen species (ROS) levels, including H2O2 and superoxide anion and malondialdehyde (MDA), but combined treatment of ethylene plus H2S alleviated oxidative stress in fruit. Furthermore, the activities of antioxidative enzymes catalase (CAT) and ascorbate peroxidase (APX) were increased by ethylene + H2S treatment in comparison with ethylene alone, whereas the activities of lipoxygenase (LOX) and polyphenol oxidase (PPO) were attenuated by H2S treatment. Further investigations showed that H2S repressed the expression of ethylene synthesis-related genes AdSAM, AdACS1, AdACS2, AdACO2, and AdACO3 and cysteine protease genes, such as AdCP1 and AdCP3. Taken together, our findings suggest that H2S alleviates kiwifruit ripening and senescence by antagonizing the effect of ethylene through reduction of oxidative stress and inhibition of ethylene synthesis pathway.

Open access

Qing Shen, Hua Bian, Hai-yan Wei, Li Liao, Zhi-yong Wang, Xiao-yan Luo, Xi-peng Ding, Zhenbang Chen and Paul Raymer

Seashore paspalum (Paspalum vaginatum) is an important warm-season turfgrass distributed in tropical and coastal areas. It has excellent resistance to abiotic stresses, such as salinity, drought, and low temperature. However, the research on genetic diversity of local P. vaginatum collections from China is limited. In this study, the genetic diversity among 58 P. vaginatum accessions from four different provinces in China and four cultivars were assessed using simple sequence repeat (SSR) markers. The results indicated that a total of 45 alleles were detected by 19 polymorphic markers, with a range of 2 to 4 and an average of 2.4 alleles per marker. The genetic similarity coefficients between each pair of the 58 P. vaginatum accessions and four cultivars ranged from 0.51 to 1.00, with an average of 0.77. The range of variation of Shannon diversity index of each SSR marker was 0.047 to 1.075, with an average of 0.486. The polymorphic information content of each SSR marker varies from 0.016 to 0.577, with an average of 0.249. The results of cluster analysis and principal component analysis (PCA) showed that 58 P. vaginatum accessions and four cultivars were divided into four groups. These results provide the theoretical basis for the genetic diversity assessments and molecular marker–assisted breeding of P. vaginatum species.