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Graft incompatibility of 3 chestnut Castanea spp. was studied. Nine American chestnut C. crenata Sieb. & Zucc and 15 Chinese chestnut C. mollissima Bl. cultivars used as scions were tested on rootstocks of Chinese chestnut. Interspecific grafts with 7 of 9 American chestnut selections had satisfactory graft compatibility on Chinese chestnut rootstocks (70%-100%), so did 6 of 8 Japanese chestnuts. Twelve and 10 out of 15 Chinese cultivars had high graft compatibility on Chinese chestnut rootstocks. Three Chinese cultivars with less than 50% success were probably due to very diverse genetic variation within one cultivar group of Chinese chestnuts. Spring grafts always had higher percent success rates than fall grafts. Graft incompatibility was not found to be related to the cambial isoperoxidase banding patterns in the present study.
Pedicularis rex C. B. Clarke ex Maxim., an endemic species with potential horticultural traits from Himalaya, has a unique cup-like petiole structure and highly infraspecific floral variation among members of the lousewort genus (Orobanchaceae). We developed 13 microsatellite markers from three microsatellite-enriched libraries (AG, AC, and AAG) of P. rex with a modified biotin–streptavidin capture technique. Polymorphism of each locus was assessed in 22 individuals with representation of five populations of P. rex. Number of alleles per locus (A) ranged from two to seven with an average of 4.38. The observed and expected heterozygosities varied from 0.03 to 0.86 and 0.45 to 0.84, respectively. Additionally, among the 13 identified microsatellite markers, 11 of them were successfully amplified in species P. thamnophila, and five of them showed polymorphisms. This study may provide important information for further investigation on the population genetics, introduction, and acclimatization of P. rex and its congeners.