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- Author or Editor: Hong Lin x
The polymerase chain reaction (PCR) and RAPD fragments are potentially useful methods for identifying turfgrass cultivar breeding lines. RAPD markers were studied in 25 vegetatively propagated buffalograss lines using oligonucleotide random primers and agarose-gel electrophoresis to determine their potential for identifying cultivar breeding lines. The variation of RAPD markers was extensive. The RAPD markers produced by one random primer were sufficient to separate the 25 buffalograss lines. Cluster analysis baaed on' the RAPD markers produced by two random primers revealed that the 25 buffalograss lines generally fell into two groups: diploid and hexaploid. Three DNA extraction methods—sarcosyl lysis-chloroform extraction-isopropanol precipitation, sodium dodecyl sulfate (SDS) lysine-isopropanol precipitation, and boiling in the presence of Chelex-100 resin—and fresh or oven-dried tissues were tested for reproducibility of RAPD markers. The three DNA extraction methods, using dry or fresh plant tissues, produced highly comparable RAPD marker profiles. More than 80%1 of the RAPD markers was consistently detected in six replicate analyses. The above studies demonstrate that small quantities (5 mg) of oven-dried leaf tissue and several DNA extraction methods can be used for buffalograss fingerprint studies.
The DNA extracted from cambium tissues of grape (Vitis spp., Muscadinia rotundifolia Small) rootstocks was found to be suitable for molecular analysis. Its quality was equivalent to that of DNA extracted from leaf tissues, although the yield was higher from leaves. The use of cambium tissue allows DNA extractions during dormancy or from grafted rootstocks where leaves are not available. The DNA extracted was suitable for restriction enzyme digestion and for analysis by restriction fragment length polymorphism (RFLP), randomly amplified polymorphic DNA (RAPD), and simple sequence repeats.
The effects of regenerant wastewater irrigation and high concentrations of Ca2+, K+, Mg2+, and Cl– on growth and ion uptake of nine species of landscape plants were studied. Significant differences in chloride tolerance were detected among the species. Generally, the species that had greater uptake of chloride grew less than species that took up less amounts of chloride. Lace fern (Athyrium filix-femina Roth.) had the highest tissue Cl concentration and was the most affected. Hydrangea (Hydrangea macrophylla Ser.) also had high tissue Cl concentration, but showed no growth reduction. Its tolerance was attributable to a high tissue Ca concentration. The data suggest that in the species tested, higher tissue Ca concentrations were positively correlated with plant tolerance to Cl. Overall, the Cl– concentration in the wastewater seems to be the factor most likely to create problems for the landscape plants. However, severe negative effects will probably be noticed only for very sensitive plant species, but it is important to determine this before applying regenerant irrigation water.
Volatile chemicals emitted from the flowers of chinese wisteria (Wisteria sinenesis) and japanese wisteria (W. floribunda) were collected using a dynamic headspace technique and identified using gas chromatography–mass spectrometry; 28 and 22 compounds were detected from chinese wisteria and japanese wisteria flowers, respectively. These chemicals can be classified into four major classes, including fatty acid derivatives, benzenoids/phenylpropanoids, terpenoids, and nitrogen-containing compounds. Two monoterpenes, (E)-β-ocimene and linalool, belonging to the class of terpenoids, were the most abundant compounds emitted from both species. Despite strong similarity, the floral volatile profiles of the two species displayed variations in both quality and quantity. Chinese wisteria was selected as a model for further study of volatile emission from different parts of flowers, emission dynamics, and regulation of floral scent production. Although floral volatiles were detected from all flower parts, petals emitted the most. The emission of floral volatiles displayed a diurnal pattern with the maximal emissions occurring during the daytime. This rhythmic pattern was determined to be light-dependent. Regulation of floral volatile emission by exogenous chemicals, including silver thiosulphate (an ethylene inhibitor), salicylic acid, and jasmonic acid, also was analyzed. Generally, jasmonic acid promoted the emission of floral volatiles. In contrast, neither silver thiosulphate nor salicylic acid showed a significant effect on floral volatile emission. The results presented in this article suggest that wisteria can serve as a useful system for exploring novel biochemistry of floral scent biosynthesis. They also build a foundation for the study of the biological/ecological significance of floral volatiles on the reproductive biology of wisteria species.
Abstract results showed that many germplasms of P. Armeniaca. P. persica and a few germplasms of P. Salicina had immersed into those of P. mume. Some cultivars (strains) possessed species characteristics coming from 2 or 3 of the 3 species in a single plant. All of the plants tested were transition types of the related varieties such as var. bungo and so on. Some new characteristics of P. mume were found in a few strains. The resources were classified into highly, mediumly and lightly backcrossed types. The mean yield index and fruit weight of highly back-crossed type (HB) were significantly higher than those of the other 2 types. The setting rate of HB was higher and significantly higher than that of lightly and mediumly type, respectively. There were no significant differences in mean bitter index and flower index among them.