Search Results
You are looking at 1 - 2 of 2 items for
- Author or Editor: Harold B. Tukey Jr x
Abstract
The ultrastructural localization of polysaccarides in Malus domestica Borkh. leaf cuticles was investigated by electron microscopy staining. The cuticle/cell wall interface was not stained by ruthenium red or hydroxylamine-ferric chloride, indicating the lack of a distinct pectin layer. The inner region of the cuticle was intensely and uniformly stained by phosphotungstic acid (PTA) and was lightly stained by silver proteinate, indicating the presence of polysaccharides, which in part may be pectin as indicated by staining with ruthenium red. Fibrils in the inner region of the cuticle were not stained by ruthenium red, hydroxylamine-ferric chloride or silver proteinate, but appeared lightly stained by PTA; this suggests by deduction that the fibrils may consist of cutinized cellulose. The outer region of the cuticle was not stained by any of the staining procedures, indicating the lack of polysaccharides.
Abstract
At either 440 or 145 μEm−2s−1, greater amounts of cuticle, cutin matrix, and wax were formed at 15° than 25°C on leaves of Brussels sprouts (Brassica oleraceae. L., Gemmifera group), but the reverse occurred on leaves of carnation (Dianthus caryophyllus L.). At either 15 or 25°, greater amounts of cuticle, cutin matrix, and wax were formed at 440 than 145 μEm−2s−1 on both species. For Brussels sprouts, at 25°/440 μEm−2s−1 and 25°/145 μEm−2s−1, large parallel wax dentrites covered the leaf surface. At 15°/440 μEm−2s−1, dentrites were smaller and morphologically less elaborate. At 15°/145 μEm−2s−1, epicuticular wax occurred as scattered rods perpendicular to the leaf surface. Carnation epicuticular waxes consistently occurred as rods, but as temperature and/or light intensity decreased, rod length decreased and density increased. There were no changes in internal cuticle ultrastructure of either species in different environments, but cuticle thickness increased as temperature and light intensity decreased. Epicuticular waxes visualized in surface view by scanning electron microscopy (SEM) were extracted by procedures for transmission electron microscopy (TEM) observation.