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Wol Soo Kim, Seong Bong Kim, Nam Hyun Song and Han Chan Lee

Dark browning of fruit skin of Japanese pear `Niitaka' (Pyrus pyriforia) is a physiological disorder during storage. Very higher rates of dark browning were observed in bagged pears, and in the orchards under higher humidity during the maturing season. Also the factors, such as higher humidity and lower temperature in storage room increase the dark browning. Dark browning is a different physiological disorder with the sugerficial scold of apples, which associated with conjugated trienes, oxidation products of alpha-farnesenes. The dark browning was controlled by dipping the harvested pears into 1,000 ppm diphenylamine (DPA) solution for 5 seconds before storage.

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Gyeong Ran Do, Ju Hee Rhee, Wan Soon Kim, Yun Im Kang, In Myung Choi, Jeom Hwa Han, Hyun Hee Han, Su Hyun Ryu and Han Chan Lee

Lilium lancifolium (syn. L. tigrinum) is the only polyploidy-complex species involving both diploid (2n = 2x = 24) and triploid (2n = 3x = 36) plants in the genus. The origin of natural triploid remains a mystery and research has been limited mainly to chromosomal studies that have overlooked research on pollen ontogeny. By spatiotemporal comparison of the development and morphology of diploid and triploid pollen grains, we study the correlations between pollen fertility and morphological development in diploid and triploid plants and propose the necessity and importance of further research on natural polyploid-ontogenetic diversity. In this comparative investigation, we used various microscopy techniques including histological analyses, scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The main morphological differences between triploid and diploid pollen grains started with abnormal tetrad formation of triploid, followed by inadequate amylogenesis and amylolysis in young microspores, and finished with the formation of an abnormal structure of pollen surface layers in maturing pollen grains, which finally resulted in pollen grain unfolding and male sterility. From observing the series of morphological events that induced male-sterile pathway in natural triploid pollens, this study showed a variety of correlations between pollen development and fertility, which differed from male sterility resulting from gene mutation, indicating that there exists greater variability in pollen male-sterile ontogeny. Our results suggest that multilateral research is required for understanding the fickle ontogeny of natural male-sterile polyploid.

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Kang Hee Cho, Seo Jun Park, Su Jin Kim, Se Hee Kim, Han Chan Lee, Mi Young Kim and Jae An Chun

Blueberry cultivars have traditionally been identified based on the evaluation of sets of morphological characters; however, distinguishing closely related cultivars remains difficult. In the present study, we developed DNA markers for the genetic fingerprinting of 45 blueberry cultivars, including 31 cultivars introduced from the United States Department of Agriculture. We obtained 210 random amplified of polymorphic DNA (RAPD) markers using 43 different primers. The number of polymorphic bands ranged from three (OPG-10 and OPQ-04) to eight (OPR-16), with an average of five. A cluster analysis performed with the unweighted pair group method using arithmetic averages produced genetic similarity values among the blueberry cultivars ranging from 0.53 to 0.85, with an average similarity of 0.68. A dendrogram clustered the 45 blueberry cultivars into two main clusters, with a similarity value of 0.65. Cluster I consisted of four rabbiteye cultivars (Pink Lemonade, Alapaha, Titan, and Vernon) and the Ashworth northern highbush cultivar. Cluster II consisted of 31 northern highbush cultivars, eight southern highbush blueberry cultivars, and Northland half-highbush blueberry cultivar. Fifty five RAPD fragments selected were sequenced to develop sequence-characterized amplified region (SCAR) markers, resulting in the successful conversion of 16 of 55 fragments into SCAR markers. An amplified polymorphic band has the same size as the RAPD fragment or smaller according to the primer combinations in the 16 SCAR markers. Among these markers, a combination of 11 SCAR markers provided sufficient polymorphisms to distinguish the blueberry cultivars investigated in this study. These newly developed markers could be a fast and reliable tool to identify blueberry cultivars.