Vigor, petiole length, stand, runner production, yield per square meter of matted row, and numbers of trusses per crown were greater in tissue culture-produced plants when compared to runner plants of strawberry (Fragaria × ananassa Duch). Leaf area and shape, number of serrations per leaflet, petiole pubescence density, flowers per truss, yield per crown, harvest time, small fruit weight, and percent imperfect fruits did not vary appreciably between propagation methods. Size of large fruit and mean fruit weight were less in tissue culture-produced plants. All changes in performance were caused, at least in part, by the increased vigor and axillary bud activity of tissue culture-produced plants. Tissue culture propagation of strawberry is particularly adapted to production of planting stock for nurseries because 60% more runner plants are produced from tissue culture plants. Production of variant plants from tissue culture was largely limited to 1 meristem-tip line. For certain cultivars, some adjustment of nursery inspection procedures for off-type plants will be required.