Early production and high energy efficiency are important in greenhouse vegetable production in cold regions. A dynamic temperature integration strategy with low pre-night temperature (PNT) has been developed to reduce energy consumption and to improve early fruit yield and energy use efficiency. However, the application of this temperature control strategy is feasible only if there is no crop yield and quality loss. To determine the low PNT tolerance threshold and explore the mechanism of this temperature control strategy on plant growth and development, the effects of four PNT temperature integration treatments (PNT9, PNT11, PNT13, and PNT15, with an actual PNT of 9.4, 11.3, 13.3, and 15.1 °C, respectively) on greenhouse tomatoes (Solanum lycopersicum) were investigated. The PNT was applied at the beginning of the night for 3 h, whereas temperatures in other periods during a day (24 h) were adjusted accordingly to ensure the same 24-h average temperature (19.4 °C) for all PNT treatments. Four cultivars (Bigdena, Clarance, Quest, and Conchita), representing all three types (beefsteak, cluster, and cherry) of greenhouse tomatoes, were used in the study. The optimum PNT for fruit yield was 13.8 and 14.9 °C for ‘Bigdena’ and ‘Conchita’, respectively. Low PNT down to 11 °C did not compromise fruit yield and plant development in ‘Clarance’, and thus a PNT lower than 13 °C can be used for ‘Clarance’ if it does not have a negative effect on fruit quality. In ‘Bigdena’ and ‘Conchita’, the above-ground biomass increased with increasing PNT at the low range of PNT, peaked at ≈13 °C PNT (13.7 and 13 °C for ‘Bigdena’ and ‘Conchita’, respectively), then declined at high PNT. Leaf photosynthesis rates were increased by the highest PNT (PNT15), whereas respiration rates were reduced by the lowest PNT (PNT9). Therefore, PNT at ≈13 °C might have allowed for the proper balance between the high photosynthesis for photoassimilate generation and the low respiration for photoassimilate conservation and thus accumulated the highest photoassimilate and the highest fruit yield in ‘Bigdena’. Flower development rate in ‘Conchita’ decreased linearly with low PNT, which might have limited the response of its fruit yield to low PNT and raised the optimum PNT for fruit yield to 14.9 °C. Temperature integration with proper low PNT can be an effective climate control strategy for increasing early fruit yield and energy use efficiency in greenhouse tomato production.