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  • Author or Editor: G.M. Blake x
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Vegetative long-shoot buds, greenwood stems, and immature needles of 20-year-old western larch (Larix occidentalis Nutt.) were cultured to induce multiple bud formation. Explants were collected year-round and cultured on a modified Schenk and Hildebrandt (SH) medium containing 6-benzyladenine (BA) at 0, 1, 5, 10, 50, or 100 μm. Multiple buds were produced on buds and stems with terminal meristems, but not on needles or stem sections. The induction of de novo buds and development of axillary buds required BA at 1 to 10 μm; higher concentrations of BA were less effective. More explants formed multiple buds on SH than on modified Murashige and Skoog (MS) media. Multiple buds formed on more buds and stems excised during the growing season than from dormant buds. Buds cultured on media containing gibberellin died within 6 weeks; auxin caused bud elongation but no multiple buds formed. Chemical names used: N-[(trichloromethyl)thio]-4-cyclohexene-1,2-dicarboximide (captan); 6-benzyladenine (BA); 1H-indole-3-butyric acid (IBA); 1H-indole-3-acetic acid (IAA); gibberellin (GA4+7).

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Abstract

‘Hull Thornless’ (Rubus sp., is a vigorous and productive thornless blackberry cultivar with firm, sweet, fruit. It is named for the late John W. (Jack) Hull, formerly of the University of Maryland, the University of Arkansas, and the U.S. Department of Agriculture, who spent most of his life breeding blackberries and raspberries. ‘Hull Thornless’ is the fifth in a series of tetraploid, genetically thornless blackberry hybrids developed by the USDA and cooperating agencies (4). It is adapted principally to USDA Plant Hardiness Zones 6-8.

Open Access