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- Author or Editor: Francisco A. Ferreira x
Six F1's involving 6 multiple genetic marker stocks and a common inbred parent (PU812) were cultured to study the genotypic effect on regeneration ability and frequency of somaclonal variation in R0 for the known heterozygous marker genes. Leaf discs 7 mm in diameter were excised from young fully expanded leaves of 6-7 week old plants, and cultured on MS medium supplemented with cytokinins (Kinetin, Benzyladenine) and IAA. With few exceptions, the parents and F1's responded similarly to different hormone combinations. The beat hormone combinations for shoot formation were 4 mg/l Kinetin + 0.5 mg/l IAA and 2.3 mg/l BA + 0-0.18 mg/l IAA.
Only 2 of the 6 multiple marker stocks and the common inbred parent (PU812) were successfully regenerated. Four of the six hybrids between PU812 and the multiple markers were readily regenerated, whereas 2 hybrids failed to regenerate with several different hormonal combinations. No mutations have been observed for the known heterozygous markers in 76 R0 tissue culture regenerants.