Aeration through subsurface drip irrigation (SDI) can promote plant growth and increase crop yield; however, more research is focused on annual crops, and there are few studies on perennial crops. We have studied a new type of SDI (SDI with tanks) suitable for cultivation and production of perennial fruit trees and photovoltaic aeration device in greenhouse. The results showed that aeration irrigation promoted the growth of new leaves, fine roots, and new branches of grape, regulated O2/CO2 content in rhizosphere soil, and accelerated air exchange in rhizosphere soil. This study showed that aeration irrigation did not change the structure of bacteria and fungi but significantly increased the abundance of aerobic bacteria, such as Nitrospira and Cytophagia. Moreover, it promoted the increase of Pseudomonas and Aspergillus related to phosphate solubilization, that of Bacillus related to potassium solubilization, and that of Fusarium related to organic matter (OM) decomposition. This study shows that aeration irrigation through SDI with tanks can promote grape growth, which may be related to the ability of aeration irrigation to change the gas composition of rhizosphere soil, optimize the structure of rhizosphere soil microorganism.