Citrus huanglongbing (HLB), or greening, is the most destructive citrus disease worldwide and is threatening the sustainability of the industry in major citrus-growing regions. Various treatments have been proposed in the literature to manage the disease. We review such literature and conduct an economic analysis based on the reported treatment effects on fruit yield and quality to identify cost-effective management strategies. Our results suggest that, among the treatments we reviewed, broad-spectrum insecticides provide the only cost-effective strategy for mitigating the impact of the disease. Our findings and discussion should help growers, policymakers, and other stakeholders make informed decisions in the search for effective, sustainable, and environmentally friendly treatments and policies against HLB.
Sheng Li, Feng Wu, Yongping Duan, Ariel Singerman and Zhengfei Guan
Ji-Lian Zheng, Lan-Ying Hu, Kang-Di Hu, Jun Wu, Feng Yang and Hua Zhang
Hydrogen sulfide (H2S) has been identified as a multifunctional signaling molecule in plants. Here, we show that H2S delayed postharvest senescence of fresh-cut apples (Malus ×pumila) in a dose-dependent manner. Exogenous H2S application maintained significantly higher levels of ascorbic acid, flavonoids, total phenolics, reducing sugars and soluble proteins, and lower levels of free amino acids in apple slices compared with controls. Further investigations showed that H2S significantly reduced the accumulation of superoxide radicals, hydrogen peroxide (H2O2) and malondialdehyde (MDA). Apple fruits fumigated with H2S contained significantly higher activities of ascorbate peroxidase (APX), catalase (CAT), glutathione reductase (GR), guaiacol peroxidase (POD) and superoxide dismutase (SOD), and lower activities of lipoxygenase (LOX), phenylalanine ammonia lyase (PAL), polyphenol oxidase (PPO), and protease relative to controls. H2S also upregulated MdDHAR expression and downregulated the expression of MdLOX2, MdPG1, MdPPO, MdACO1, MdERS1, and MdETR1 in postharvest apple tissue. The present study indicates that H2S was involved in delaying postharvest senescence of apples by acting as an antioxidant and by regulating senescence-related gene expression.
Wenlei Guo, Li Feng, Dandan Wu, Chun Zhang and Xingshan Tian
Widespread herbicide-resistant weeds and severe insect pest infestations pose a challenge to the preplant pest management (PPPM) strategy currently in use in leaf vegetable fields in southern China. The aim of this study was to develop a new weed and insect control method for use before planting leaf vegetables in southern China. Two flaming machines (a tractor mounted and a trolley flaming machine) were designed, and their efficacies for the control of insect and weed pests were evaluated and compared in two field trials. With liquefied petroleum gas (LPG) at 101 kg·ha−1, flaming machines reduced plant numbers by 86.7% to 98.8% 2 days after treatment (DAT), which was equal to or higher than the reduction after application of paraquat at 900 g·ha−1. Some weed species, especially awnless barnyard grass (Echinochloa colona) and goosegrass (Eleusine indica), regrew at 7 DAT, resulting in a decrease in control efficacy. Flaming machines also reduced the number of diamondback moth (Plutella xylostella) larvae by 83.0% to 88.2% and the number of adult striped flea beetles (Phyllotreta striolata) by 64.9% to 80.9%. This is the first report on flaming treatment in China to show that this method is a promising alternative to chemical pesticides for PPPM in leaf vegetable fields.
Feng Gao, Arvind H. Hirani, Jun Liu, Zheng Liu, Guohua Fu, Chunren Wu, Peter B.E. McVetty and Genyi Li
There are various clubroot pathogen (Plasmodiophora brassicae) resistance genes within Brassica species with european turnip (B. rapa ssp. rapifera) being identified as potentially the best source of resistance for the development of clubroot-resistant cultivars in chinese cabbage (B. rapa ssp. pekinensis). To use clubroot resistance genes effectively, it is necessary to map these genes so that molecular markers inside or closely linked to these resistance genes can be developed. Using molecular marker-assisted selection, the clubroot resistance genes can be effectively transferred from cultivar to cultivar and from species to species. In this report, one clubroot resistance locus was mapped on linkage group A3 using five segregating populations developed from five chinese cabbage cultivars, suggesting that all the five cultivars shared the same clubroot resistance locus. Furthermore, one of these five chinese cabbage cultivars was used to develop a large segregating population to fine-map this clubroot resistance locus to a 187-kilobp chromosomal region. Molecular markers that are closely linked to the mapped clubroot resistance locus have been developed that can be used for marker-assisted selection in chinese cabbage and canola/rapeseed (B. rapa and B. napus) breeding programs.
Long-na Li, Songjun Zeng, Feng Zheng, Zhi-lin Chen, Kun-lin Wu, Jian-xia Zhang and June Duan
Ten polymorphic microsatellite loci were isolated and characterized from an enriched genomic library of Paphiopedilum concolor (Batem.) Pfitzer. The number of alleles per microsatellite locus ranged from three to 11 with an average of 6.4 in a sample of 30 individuals from three populations. The observed and expected heterozygosity ranged from 0.200 to 0.800 and from 0.544 to 0.827, respectively. These microsatellites can be used as tools to investigate the genetic structure of P. concolor populations and relationship patterns with closely related taxa.