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Jameel M. Al-Khayri, Feng H. Huang and Teddy E. Morelock

Callus, induced in the dark from leaf tissue of spinach (Spinacia oleracea L. cv. Fall Green) on Murashige and Skoog (MS) medium supplemented with (in mg·liter -1) 2 kinetin and 0.5 2,4-D regenerated shoots upon transfer to a medium containing 2 kinetin, 0.01 2,4-D, and 1 GA3. Complete plants were established by stimulating rooting of the shoots with 1 mg IBA/liter and transferring them to potting soil; survival was 60%. Chemical names used: N-(2-furanylmethyl)-1H-purin-6-amine (kinetin); 2;4-dichlorophenoxy acetic acid (2,4-D); gibberellic acid (GA3); 1H-indole-3-butanoic acid (IBA).

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Jameel M. Al-Khayri, Feng H. Huang, Teddy E. Morelock and Tahani A. Busharar

A preliminary study has shown that the addition of 15% (v/v) coconut water (CW) to the culture medium significantly improved callus growth, shoot-regenerative capacity, and shoot growth in leaf disk cultures of spinach (Spinacia oleracea L.). Subsequently, the influence of a range of CW concentrations, 0%, 5%, 10%, 15%, or 20% (v/v), was examined. Callus weight obtained after 5 weeks showed direct relationship to the concentration of CW. This stimulator action was observed in both cultivars tested in this study, `High Pack' and `Baker'. On CW-containing medium, shoot regeneration was expedited to 4 to 5 weeks compared with 8 to 12 weeks on a CW-free medium. Callus of `Baker' induced on a CW-free medium exhibited a significant increase in shoot regeneration frequency when transferred to a regeneration medium enriched with CW, suggesting that the addition of CW to the regeneration medium only is sufficient to achieve improved regeneration.

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Jameel M. Al-Khayri, Feng H. Huang, Teddy E. Morelock and Forrest E. Lane