Twelve cacao (Theobroma cacao) clones propagated by grafting and orthotropic rooted cuttings of somatic embryo-derived plants were grown on an Ultisol soil at Corozal, Puerto Rico, and evaluated for 6 years of production under intensive management. Year, variety, year × variety, and propagation treatment × variety interactions indicated significant effects for dry bean yield, number of pods produced, pod index, plant height, and stem diameter. Propagation treatments had a significant effect on dry bean yield and pod index but not on number of pods produced. Average yield across varieties for both propagation treatments was 2087.9 kg·ha−1 per year of dry beans. There was a highly significant variety effect. ‘UF-668’ was the top yielder averaging 2536.7 kg·ha−1 per year of dry beans; however, this yield was not significantly different from the average yield of varieties ‘TARS-30’, ‘TARS-1’, ‘TARS-13’, ‘TARS-14’, and ‘TARS-2’, which averaged 2427.0 kg·ha−1 per year. Except for ‘UF-668’, the TARS varieties were released in 2009 as high-yielding selections. Propagation treatments had a significant effect on dry bean yield. Dry bean yield of varieties propagated by grafting was 7% higher (2166.7 kg·ha−1 per year) than those propagated by orthotropic rooted cuttings of somatic embryo-derived plants (2009.2 kg·ha−1 per year). This yield difference could not be attributed to grafted plants being more vigorous nor by differences in root architecture. The lowest pod index value in both propagation treatments was obtained by ‘UF-668’; however, pod index for this variety did not differ significantly from values for ‘TARS-2’ and ‘TARS-23’ in grafted plants and from ‘TARS-2’, ‘TARS-23’, and ‘TARS-1’ in plants propagated by orthotropic rooted cuttings of somatic embryo-derived plants. With few exceptions, flavor characteristics were not significantly affected by propagation treatments. Although there were significant differences between plant propagation treatments for some of the variables measured in this study, these were not of a magnitude that would preclude the use of somatic embryogenesis as a viable propagation system for cacao.
Ricardo Goenaga, Mark Guiltinan, Siela Maximova, Ed Seguine and Heber Irizarry
Harry C. Bittenbender, Loren D. Gautz, Ed Seguine and Jason L. Myers
Standardized and repeatable techniques for microfermentation and drying small samples (<100 g) of cacao (Theobroma cacao), also known as cocoa, are necessary to identify new varieties having high yield and quality. Sensory analyses of the processed cacao seed (bean) are a critical component to develop varieties for Hawaii’s cacao to chocolate industry. A microfermentation and drying system capable of processing multiple samples of mucilage-covered cacao beans ranging from 60 to 6000 g was developed. The effects of fermentation variables, genetic background, management, site, and season on quantitative and qualitative attributes can be studied using this protocol. Beans processed using the College of Tropical Agriculture and Human Resources (CTAHR) bag system (CBS) are inoculated with microorganisms on their fruit (pod) surface. This enables a better assessment of the terroir of sites when the dried fermented beans are roasted and processed. Clean, inexpensive, disposable polyethylene bags serve as fermentation vessels. The fermentery has a temperature controller that follows a fermentation temperature profile. Sun drying is replaced by drying in the laboratory. Two-month storage in ambient outdoor humidity and temperature completes the protocol. The CBS is an improvement to existing cacao microfermentation methods because beans from single pods can be fermented. No microbial isolates, inoculums, or foreign pulp from other trees and sites are used. Less labor is required to maintain the fermentation. In laboratory drying is less variable than sun drying. The CBS is a flexible and reliable method to microferment cacao for scientists, small growers, and hobbyists.