A study was initiated in November, 2002 to determine the effects of exposing two Southern Highbush blueberries (Vaccinium corybosum L) to artificial chilling hours on initiation of bud break and advancement of floral and vegetative bud maturity. Plants of `Jubilee' and `Misty' were divided into 2 groups in which one was left outdoors, allowing chilling to occur and accumulate naturally, while the other group was placed in a growth chamber set at a constant artificial temperature of 4 °C. Five plants of each cultivar were then placed into a heated greenhouse after 0, 200, 400, 600, or 800 hours of chilling (total hours of exposure to <5 °C) had accumulated for forcing of flower bud development. The progression of floral bud development of the terminal three buds on five tagged stems was observed at 7-10 day intervals for 30 days. At the end of the forcing period observations were also made on total percent vegetative and floral bud break. Prior to accumulating sufficient chilling requirements, chilling delivery method did not appear to influence the rate of floral bud development since none advanced past stage 3 regardless of chilling regime used. However after chilling requirements were met, flower buds of plants that were allowed to chill naturally developed more quickly than did those chilled by artificial means.
Donna A. Marshall*, Stephen J. Stringer and James M. Spiers
Donna A. Marshall, James M. Spiers and Stephen J. Stringer
To improve the quality of berries during handling and shipping, blueberry breeders have strived to develop a fruit that is firm in texture. However, some previous studies have suggested that blueberry cultivars with firmer fruit were more susceptible to splitting. This study was conducted to further investigate the correlation between splitting susceptibility and fruit firmness. Various cultivars and selections of rabbiteye (Vaccinium ashei) and southern highbush (interspecific hybrids primarily consisting of V. corymbosum) blueberry were used to determine whether berries displaying higher fruit firmness also have a higher incidence of splitting. Three distinctly different measurements of berry firmness were obtained using two instruments: QTS25 and FirmTech2. Berries were subsequently submitted to laboratory procedures to induce splitting. In general, firmness measured as either deformation (FirmTech2) or modulus of elasticity (QTS25) correlated with splitting tendencies. There are exceptions, however, that need to be further examined.
Stephen J. Stringer, Donna A. Marshall and Dennis J. Gray
Blair J. Sampson, Stephen J. Stringer and Donna A. Marshall
We evaluated relationships between floral traits of 23 genotypes of southern blueberries and indices of pollination efficiency (fruit set, fruit abortion, seed number, and berry size) for Osmia ribifloris Cockerell, a manageable solitary bee. Flower size in Vaccinium and presumably ovary size were proportional to berry size, except for the tiny blooms of one V. tenellum clone (NC7808), which produce large commercial-sized berries of ≈2 g. Longer-styled blueberry flowers visited by O. ribifloris produced the heaviest berries with the most seeds. Osmia ribifloris reliably pollinated ‘Climax’ and ‘Tifblue’ rabbiteye blueberries. However, the peculiarly misshapen blooms of ‘Premier’ rabbiteye blueberry receive less pollination from O. ribifloris and yield berries containing 25% fewer seeds. Fruit set for these misshapen ‘Premier’ flowers was equivalent to that of intact flowers indicating that this floral polymorphism would not greatly alter cultivar performance. For seven Vaccinium species, wild and cultivated alike, 80% to 100% of a plant’s fruit production depends on efficient cross-pollination by bees such as O. ribifloris.
Donna A. Marshall, James M. Spiers and Kenneth J. Curry
Calcium is commonly known to affect the developmental processes of many plants, and its role as a major nutrient has been interpreted in terms of its interaction with components of the cell wall and membrane. A 2-year study was conducted to assess the affects of calcium foliar feed fertilization applied at bloom and throughout floral development on the reduction of rain-related splitting in blueberries. Foliar-applied calcium at 0.2% or 0.9% concentration did not successfully decrease splitting in blueberries to a statistical and, more importantly, an economically significant level. Calcium sprays also had no adverse affects on the fruit firmness, quality, or calcium concentrations within the fruit.
Stephen J. Stringer, Penelope Perkins-Veazie and Donna A. Marshall
The consumption of fresh fruit and vegetables has always been known to provide essential nutrition to mankind and, both anecdotally and clinically, has been linked to the prevention or alleviation of chronic diseases. The muscadine grape, a fruit native to the southeastern U.S., contains numerous phenolic compounds that act as antioxidants and also other compounds, such as resveratrol, that acts as a chemopreventative. The concentrations of these compounds present in the muscadine grape equal or exceed that known for any other small fruit. Fruit of selected muscadine grape genotypes, including breeding lines and cultivars, were evaluated over a 2-year period to assess the existing genetic base for these nutraceutical compounds. Results demonstrated that concentrations of total phenolics, ellagic acid, and resveratrol differ significantly among cultivars and breeding lines. These results suggest that it should be possible to breed for increased concentrations of the health-promoting compounds in muscadine grapes.
Donna A. Marshall, James M. Spiers and Kenneth J. Curry
Split-resistant and split-susceptible rabbiteye blueberry fruit were evaluated at all stages of development to determine “water uptake thresholds” by soaking in distilled water. Weight increase after soaking was measured, and percent weight gain was calculated to take into consideration the weight increase of the fruit from development. The ratio of percent increase in volume to weight increase resulting from water uptake was calculated. Ratios of percent water uptake to weight increase between split-susceptible ‘Tifblue’ and split-resistant ‘Premier’ blueberries were found to be similar. The split-susceptible ‘Tifblue’ had a 1.6 g/50 fruit increase with a 1.7% water uptake and a ratio of 1.08. ‘Premier’ had a higher weight increase with 3.3 g/50 fruit and also a higher percentage of water uptake at 3.6% providing a ratio of 1.09. Although both absorbed water at a constant rate shown by a linear increase of weight increase over time, ‘Premier’ absorbed a significantly greater amount of water than did ‘Tifblue’ yet remained intact and did not split.
Donna A. Marshall, James M. Spiers, Stephen J. Stringer and Kenneth J. Curry
Preharvest rainfall that occurs when fruit are fully ripe or approaching full ripeness can result in detrimental fruit splitting in rabbiteye and southern highbush blueberries. This study was initiated to develop a laboratory method to model rain-related incidence of splitting in cultivated blueberries with the goal of predicting the incidence of splitting in blueberry cultivars and selections. Multiyear field surveys of rabbiteye and southern highbush cultivars show that the incidence of rain-related splitting is strongly cultivar-dependent. Laboratory values for forced splitting and naturally occurring rain-related field splitting data show a strong correlation indicating that the incidence of fruit splitting can be accurately estimated by this laboratory method. Soaking the berries in distilled water 14 h at room temperature gives a confident determination of splitting tendencies. Blueberry breeders and geneticists can use this method to evaluate new potential blueberry cultivars for splitting tendencies as part of routine screening. This would lead to a long-term goal of reducing splitting susceptible blueberry cultivars in commercial plantings.
Donna A. Marshall, J.M. Spiers, K.J. Curry and S.J. Stringer
Fruit splitting takes place in rabbiteye and southern highbush blueberries when a preharvest rainfall occurs and when fruit are fully ripe or approaching full ripeness. This study was initiated to develop a laboratory method to identify the rain-related incidence of splitting in cultivated blueberries. Multi-year field surveys of select rabbiteye and southern highbush cultivars show that the incidence of rain-related splitting is strongly cultivar dependent. Year to year variations within cultivars reflected yearly differences in ripening times and amounts and timing of rainfall. Laboratory values of forced splitting and field splitting data of three years show a strong correlation indicating that the incidence of fruit splitting can be accurately estimated by laboratory methods. Soaking the berries in distilled water 14 hours at room temperature gives a confident determination of splitting tendencies. Blueberry breeders can use this method to evaluate new potential blueberry cultivars for splitting tendencies. This laboratory method could also be used by geneticists to test selections accurately for splitting tendencies as part of routine screening. This can lead to a long-term goal of reducing splitting susceptible blueberries in commercial plantings.