Growth-chamber studies were conducted to examine the ability of seven vegetable crops-`Blue Lake' bean (Phaseolus vulgaris L.), `Detroit Dark Red' beet (Beta vulgaris L.), `Burgundy' okra (Abelmoschus esculentus (Moench), `Little Marvel' pea (Pisum sativum L.), `California Wonder' bell pepper (Capsicum annuum L.), `New Zealand' spinach (Spinacia oleracea L.), and `Beefsteak' tomato (Lycopersicon esculentum Mill.)–to adjust osmotically in response to water-deficit stress. Water stress was imposed by withholding water for 3 days, and the adjustment of leaf and root osmotic potentials upon relief of the stress and rehydration were monitored with thermocouple psychrometers. Despite similar reductions in leaf water potential and stomata1 conductance among the species studied, crop-specific differences were observed in leaf and root osmotic adjustment. Leaf osmotic adjustment was observed for bean, pepper, and tomato following water-deficit stress. Root osmotic adjustment was significant in bean, okra, pea, and tomato. Furthermore, differences in leaf and root osmotic adjustment were also observed among five tomato cultivars. Leaf osmotic adjustment was not associated with the maintenance of leaf growth following water-deficit stress, since leaf expansion of water-stressed bean and pepper, two species capable of osmotic adjustment, was similar to that of spinach, which exhibited no leaf osmotic adjustment.
Stan D. Wullschleger and Derrick M. Oosterhuis
Mengmeng Gu, Curt R. Rom, James A. Robbins, and Derrick M. Oosterhuis
Water was withheld from 2-year-old seedlings or rooted cuttings of four birch genotypes (Betula alleghaniensis Britton, B. davurica Pall., B. nigra L. ‘Cully’, and B. papyrifera Marsh.) until the combined weight of the container and plant decreased below 40% of its original value to induce plant predawn water potential between −1.5 MPa and −2.1 MPa, after which plants were supplied with a requisite amount of water to reach 40% of its original value for 5 weeks under controlled conditions to investigate changes in gas exchange, osmotic solutes, leaf abscission, and growth compared with well-watered (WW) plants. Observations indicated that three of the four genotypes (except B. papyrifera) expressed three stages of photosynthetic response during water deficit: 1) a stress stage, 2) an acclimation stage, and 3) an adapted (or tolerance) stage. The stages were characterized by decreasing, increasing, and stabilized Pnws/ww (net photosynthesis presented as a ratio of water-deficit stressed (WS) plants to WW plants), respectively. A strong relationship between Pn and g S observed in the WS plants of the four genotypes, suggested inhibition of Pn by stomatal closure. After exposure to water deficit for 5 weeks, Pnws/ww recovered to 70% of the initial value for B. alleghaniensis and B. nigra ‘Cully’ and 98% for B. davurica and B. papyrifera. WS plants had higher foliar concentrations of chlorophyll a and b (nmol/g) and potassium (%) than the WW plants. Increased levels of polyols (mg/g) were detected only in the WS plants of B. allegahaniensis. Increased levels of carbohydrates or organic acid under water deficit were not detected. A significant increase in leaf abscission in the WS plants of B. papyrifera compared with the other genotypes could be a morphological adaptation to water deficit conditions and facilitate recovery of Pnws/ww during the acclimation stage.