Verticillium wilt, caused by the soilborne fungus Verticillium dahliae, is a significant disease affecting watermelon (Citrullus lanatus) production in Washington State. This field study at three locations in Washington in 2015 compared verticillium wilt susceptibility, fruit yield and quality of nongrafted watermelon, and grafted plants grown with black plastic and clear plastic mulch. Overall for grafting treatments, area under disease progress curve (AUDPC) values were higher for nongrafted ‘TriX Palomar’ (765) than for ‘TriX Palomar’ grafted onto ‘Super Shintosa’ (132), ‘Tetsukabuto’ (178), or ‘Just’ (187). Overall for mulch, the AUDPC value was higher for plants grown with black plastic mulch (385) than for plants grown with clear plastic mulch (237). Overall for location, the AUDPC value was lowest at Eltopia (84), intermediate at Othello (182), and highest at Mount Vernon (680). At season end, more Verticillium microsclerotia were present in stems of nongrafted ‘TriX Palomar’ than in grafted treatment stems at Eltopia and Mount Vernon, but not at Othello. Differences in microsclerotia presence occurred only in the top or scion portion of the stem, but not in the graft union, rootstock, or crown portions of the stem. There was no difference due to mulch in regard to Verticillium microsclerotia detected in stem assays. After harvest, V. dahliae soil density under black plastic mulch increased 6-fold at Eltopia, 4.7-fold at Othello, and 1.9-fold at Mount Vernon. In contrast, V. dahiae soil density under clear plastic mulch was nearly identical to the level at planting at each location (<1, 2.6, and 27 cfu/g at Eltopia, Othello, and Mount Vernon, respectively). There was a significant interaction between grafting and location for fruit yield such that there was no difference at Eltopia and Othello, but at Mount Vernon, yield of nongrafted ‘TriX Palomar’ was lower (7.4 kg/plant) than for grafted plants (average 13.0 kg/plant). The number and weight of marketable fruit per plant were higher at Othello (4.0 and 27.65 kg/plant, respectively) than at Eltopia (2.0 and 12.23 kg/plant, respectively) and Mount Vernon (2.2 and 11.63 kg/plant, respectively). Fruit firmness was greater overall for all three grafted treatments (average 2.67 N) than for nongrafted ‘TriX Palomar’ (2.20 N), but there was no difference in total soluble solids (TSS) or lycopene content of fruit due to grafting. Yield, fruit firmness, and TSS did not differ due to mulch type; however, lycopene content was greater for plants grown with black plastic mulch than with clear plastic mulch at Eltopia. There was no difference in TSS due to location, but fruit firmness was lower at Eltopia and Othello (2.20 and 2.44 N, respectively) than at Mount Vernon (3.00 N), whereas lycopene content was less at Mount Vernon (27.85 μg·g−1) than at Eltopia or Othello (38.58 and 36.54 μg·g−1). The results of this study indicate that although verticillium wilt symptoms were visible in watermelon plants when V. dahliae level was <3 cfu/g of soil, watermelon yield was not reduced. However, when V. dahliae soil density was >50 cfu/g of soil, yield was greater for grafted plants and for plants grown with clear plastic mulch.
Sahar Dabirian, Debra Inglis, and Carol A. Miles
Jesse Wimer, Debra Inglis, and Carol Miles
Grafting watermelon (Citrullus lanatus Thunb.) onto resistant rootstocks is used in many areas of the world to overcome soilborne disease losses including verticillium wilt caused by Verticillium dahliae Kleb. Currently, this disease poses a serious risk to watermelon growers in Washington State. To identify resistant rootstocks, the verticillium wilt reactions (chlorosis, necrosis, and wilting) of 14 nongrafted PI accessions including Benincasa hispida Thunb., Cucurbita moschata Duchesne ex Poir., and Lagenaria siceraria Molina Standl. from the U.S. Department of Agriculture National Plant Germplasm System (USDA NPGS); 11 nongrafted commercially available rootstocks; and, nongrafted ‘Sugar Baby’ watermelon (verticillium wilt–susceptible control) were visually assessed in a field naturally infested with V. dahliae at a rate of 17 colony-forming units (cfu) per gram of soil. Typical symptoms of verticillium wilt were observed on all entries. ‘Sugar Baby’ had the highest relative area under disease progress curve (RAUDPC) value (26.80), which was not significantly different from ‘64-19 RZ’, ‘Marvel’, PI 368638, PI 634982, and PI 642045 (average = 10.16). PI 419060 (1.46) had the lowest RAUDPC value, which was not significantly different from ‘Miniature Bottle Gourd’, PI 326320, PI 419016, PI 536494, PI 636137, ‘Strong Tosa’, ‘Strongtosa’, and ‘TZ 148’ (average = 3.36). The mean RAUDPC value of PI accessions (5.49) did not differ significantly from the mean value of the commercial rootstocks (5.68). Microsclerotia typical of Verticillium spp. were observed in the stems of all but one entry (PI 181913). In a greenhouse study, a subset of 12 entries were inoculated with V. dahliae, and by 22 days after inoculation (DAI), ‘Sugar Baby’ had a significantly higher disease rating than all entries except PI 419060, PI 438548, and ‘Titan’. A strong positive correlation was observed between the field and greenhouse studies. Results indicate that commercial rootstocks as well as PI accessions could be used to successfully manage verticillium wilt in Washington; however, grafting compatibility with watermelon must first be ascertained for the promising PI accessions. Although greenhouse-based verticillium wilt assays can be used to help predict rootstock performance in the field, accurate assessment may require manipulating environmental conditions (e.g., temperature and humidity) to approximate field conditions.
Sacha Buller, Debra Inglis, and Carol Miles
Growth, fruit yield and quality, and potential tolerance to verticillium wilt (Verticillium dahliae) were compared among non-grafted, self-grafted, and grafted triploid watermelon (Citrullus lanatus Thunb., ‘Crisp’n Sweet’) and heirloom tomato (Solanum lycopersicum, ‘Cherokee Purple’). Rootstocks for watermelon were ‘Emphasis’ bottle gourd (Lagenaria sicerarea) and ‘Strong Tosa’ interspecific squash hybrid (Cucurbita maxima × Cucurbita moschata), and rootstocks for tomato were ‘Beaufort’ and ‘Maxifort’ interspecific tomato (Solanum lycopersicum × Solanum habrochaites). Field trials were carried out in 2010 and 2011 at Hermiston and Eltopia (eastern Oregon and Washington, respectively) and Mount Vernon (western Washington). Grafted watermelon had significantly larger stem diameter than non-grafted and self-grafted plants both years at Mount Vernon, whereas there were no differences at Hermiston or Eltopia. Grafted tomato in 2011 had significantly larger stem diameter than non-grafted and self-grafted plants at Eltopia and Mount Vernon, and ‘Beaufort’-grafted plants were significantly taller than other treatments at Mount Vernon. Grafting did not impact watermelon or tomato fruit yield or quality at any location either year. Foliar symptoms of verticillium were not observed on ‘Crisp’n Sweet’ watermelon at the eastern locations either year; however, at Mount Vernon, ‘Emphasis’ and ‘Strong Tosa’-grafted plants had significantly lower verticillium wilt severity than non-grafted and self-grafted plants both years. Microsclerotia were observed in all recovered watermelon stems sampled at Eltopia and Mount Vernon. V. dahliae was isolated from non-grafted and ‘Emphasis’-grafted ‘Crisp’n Sweet’ stems at Eltopia and non-grafted, self-grafted, and ‘Strong Tosa’-grafted stems at Mount Vernon. Foliar symptoms of verticillium wilt and microsclerotia in stems were not observed on ‘Cherokee Purple’ plants at either location both years despite site histories of the disease. Grafting with ‘Emphasis’ and ‘Strong Tosa’ rootstocks may be an effective strategy for managing verticillium wilt on watermelon in western Washington; however, grafting ‘Cherokee Purple’ onto ‘Beaufort’ and ‘Maxifort’ did not provide any advantages for tomato under the field conditions of this study.
Jesse Wimer, Debra Inglis, and Carol Miles
Verticillium wilt caused by Verticillium dahliae is a serious disease for watermelon growers in Washington State. Grafting represents a possible alternative disease management strategy, but little is known about rootstock resistance to verticillium wilt or the performance of grafted watermelon in the different production regions of the state. In this study, verticillium wilt severity, yield, and fruit quality were evaluated at three contrasting field sites in Washington using verticillium wilt-susceptible ‘Sugar Baby’ (diploid) watermelon grafted onto four commercial rootstock cultivars (Marvel, Rampart, Tetsukabuto, and Titan); nongrafted ‘Sugar Baby’ was included as the control. Verticillium dahliae soil densities varied at each site (<1.0, 5.7, and 18.0 colony-forming units (cfu)/g soil at Othello, Eltopia, and Mount Vernon, respectively). Area under disease progress curve (AUDPC) values differed significantly among treatments at Eltopia and Mount Vernon. Nongrafted ‘Sugar Baby’ had the highest AUDPC value at all three sites, while ‘Sugar Baby’ grafted onto ‘Tetsukabuto’ had the lowest AUDPC value at Eltopia and Mount Vernon. Nongrafted ‘Sugar Baby’ also had the lowest fruit weight per plant at all sites, but ‘Sugar Baby’ grafted onto ‘Tetsukabuto’ had the highest fruit weight per plant at Eltopia and Mount Vernon. Marketable fruit weight per plant did not differ among treatments at Othello. Yield was negatively correlated with AUDPC values at both Eltopia and Mount Vernon. Fruit number per plant was only significantly impacted at Eltopia, where ‘Sugar Baby’ grafted onto ‘Tetsukabuto’ had more fruit per plant than all other treatments except ‘Sugar Baby’ grafted onto ‘Rampart’. Fruit quality (flesh firmness, total soluble solids, and lycopene content) was unaffected by grafting at either Othello or Eltopia, except for increased flesh firmness for ‘Sugar Baby’ grafted onto ‘Marvel’ and ‘Titan’ as compared with nongrafted ‘Sugar Baby’ at Eltopia. At season’s end, plants were sampled from all treatments at Eltopia and Mount Vernon and assayed for V. dahliae. Microsclerotia typical of this organism were observed in all samples. Results from this study indicate that verticillium wilt of watermelon can be successfully managed by grafting when the V. dahliae soil density exceeds 5.0 cfu/g in Washington. In addition, grafting does not reduce fruit quality and using certain rootstocks can improve the quality of flesh firmness at certain locations.
Jeremy S. Cowan, Debra A. Inglis, and Carol A. Miles
Three potentially biodegradable plastic mulch products, Mater-bi®-based black film (BioAgri), experimental polyhydroxyalkanoate film (Crown 1), and experimental spunbonded polylactic acid fabric (SB-PLA-11), were evaluated over two broccoli (Brassica oleracea var. italica) growing seasons to determine deterioration before and after soil incorporation. Pretillage mulch deterioration was evaluated in both growing seasons by rating the percent visual deterioration (PVD). Crown 1 had the greatest PVD throughout the study (P ≤ 0.05) and BioAgri also had significant pretillage deterioration. SB-PLA-11 showed no appreciable deterioration based on PVD (<1.3%) in either growing season. Postincorporation mulch deterioration was measured for 13 months after rototilling at the end of the first growing season. The average fragment area of all mulch products decreased over time after soil incorporation. The number of postincorporation mulch fragments initially increased for all mulch products, with Crown 1 and BioAgri reaching maximum fragment counts 132 and 299 days after incorporation, respectively. As the number of fragments declined, the average area of fragments did not change, suggesting that a threshold fragment size may exist at which biodegradation accelerates. At the end of the study period, 397 days after soil incorporation, Crown 1 and BioAgri had deteriorated 100% and 65%, respectively; whereas SB-PLA-11 showed very little deterioration.
Jeremy S. Cowan, Arnold M. Saxton, Hang Liu, Karen K. Leonas, Debra Inglis, and Carol A. Miles
The functionality of biodegradable mulch can be evaluated in agricultural field settings by visually assessing mulch intactness over time (a measure of deterioration), but it is unclear if mulch deterioration is indicative of mulch degradation as measured by mechanical properties (like breaking force and elongation). This 3-year study (2010–12) examined mulch percent visual deterioration (PVD) during the summer growing season in open-field and high tunnel production systems, and compared these to mulch mechanical properties at mulch installation (12–30 May), midseason (22 July–9 Aug.), and season end (6–25 Oct.), to determine if the field-based measures reliably predict degradation as revealed by changes in mulch mechanical properties. Four different types of biodegradable mulches [two plastic film mulches marketed as biodegradable (BioAgri and BioTelo); one fully biodegradable paper mulch (WeedGuardPlus); and, one experimental spunbonded plastic mulch designed to biodegrade (SBPLA)] were evaluated against a standard nonbiodegradable polyethylene (PE) mulch where tomato (Solanum lycopersicum L. cv. Celebrity) was planted as the model crop. Each year for the 3 years, PVD increased earlier for WeedGuardPlus than the other mulches in both the high tunnel and open field, and WeedGuardPlus had the greatest PVD in both high tunnels and the open field (6% and 48%, respectively). Mechanical strength of WeedGuardPlus also declined by the end of the season both in the high tunnel (up to 46% reduction) and in the open field (up to 81% reduction). PVD of BioAgri and BioTelo reached a maximum of 3% in the high tunnel and 28% in the open field by the end of the season. Mechanical strength of BioAgri and BioTelo did not change over the course of the season in either the open field or high tunnel, even though the ability of these mulches to elongate or stretch declined 89% in the open field and 82% in the high tunnel. SBPLA and PE mulches did not show a change in PVD or mechanical properties in either the high tunnel or the open field. Overall, PVD was three to six times greater by midseason in the open field than in the high tunnels. Although there were significant relationships between visual assessments and various mechanical properties for each mulch except SBPLA, the relationships differed for each mulch when evaluated separately and had coefficients of determination (R 2) below 30%. Furthermore, PVD overestimated mechanical deterioration of BioAgri and BioTelo. Results of this study indicate that mulch visual assessments may reflect general trends in changes in certain mechanical properties of the mulch; however, visual assessment and mechanical properties provide different information on deterioration. Each should be used as needed, but not as a substitute for each other.
Shuresh Ghimire, Annette L. Wszelaki, Jenny C. Moore, Debra Ann Inglis, and Carol Miles
The use of plastic biodegradable mulch (BDM) in many vegetable crops such as tomato (Solanum lycopersicum L.), broccoli (Brassica oleracea L. var. italica), and pepper (Capsicum annuum L.) has been proven to be of equal benefit as polyethylene (PE) mulch. However, there are limited research findings on the performance of BDM with a large fruited crop such as pumpkin (Cucurbita pepo L.) where the fruit can rest directly on the mulch for an extended period. To investigate whether heavy fruit might cause the mulch to degrade more quickly than expected, thereby, influencing weed control, fruit yield, and fruit quality including mulch adhesion on fruit, we carried out a field experiment in 2015 and 2016 at two locations in the United States with distinctive climates, Mount Vernon, WA and Knoxville, TN. Three plastic mulches marketed as biodegradable (BioAgri, Organix, and Naturecycle), one fully biodegradable paper mulch (WeedGuardPlus), and one experimental plastic BDM consisting of polylactic acid and polyhydroxyalkanoates (Exp. PLA/PHA) were evaluated against PE mulch and bare ground where ‘Cinnamon Girl’ pie pumpkin was the test crop. There was significant weed pressure in the bare ground plots at both locations over both years, indicating viable weed seed banks at the field sites. Even so, weed pressure was minimal across mulch treatments at both locations over both years because the mulches remained sufficiently intact during the growing season. The exceptions were Naturecycle in 2015 at both locations because of the splitting of the mulch and consequently higher percent soil exposure (PSE), and the penetration of all the plastic mulches at Knoxville by nutsedge (Cyperus sp. L.); nutsedge did not penetrate WeedGuardPlus. At Mount Vernon, overall pumpkin yield across both years averaged 18.1 t·ha−1, and pumpkin yield was the greatest with PE, Exp. PLA/PHA, BioAgri, and Naturecycle (19.9–22.8 t·ha−1), intermediate with Organix and WeedGuardPlus (15.3–18.4 t·ha−1), and the lowest for bare ground (8.7 t·ha−1). At Knoxville, overall pumpkin yield across both years averaged 17.7 t·ha−1, and pumpkin yield did not differ because of treatment (15.3–20.4 t·ha−1). The differences in yield between treatments at Mount Vernon were likely because of differences in the soil temperature. At 10 cm depth, the average soil temperature was 1 °C lower for bare ground and WeedGuardPlus as compared with PE mulch and plastic BDMs (20.8 °C). In contrast, soil temperatures were generally higher (25.2 to 28.3 °C) for all treatments at Knoxville and more favorable to crop yield compared with Mount Vernon. Forty-two percent to 59% of pumpkin fruit had mulch adhesion at harvest at Mount Vernon, whereas only 3% to 12% of fruit had mulch adhesion at Knoxville. This difference was because of the location of fruit set—at Mount Vernon, most of the fruit set was on the mulch whereas at Knoxville, vine growth was more extensive and fruit set was mostly in row alleys. Fruit quality differences among treatments were minimal during storage across both locations and years except for total soluble solids (TSS) in 2016, which was lower for bare ground and WeedGuardPlus compared with all the plastic mulches. Taken overall, these results indicate that pie pumpkin grown with BDM has fruit yield and quality comparable to PE mulch; however, adhesion of some BDMs on fruit could affect marketable yield. Furthermore, paper mulch appears to prevent nutsedge penetration.
Carol Miles, Russ Wallace, Annette Wszelaki, Jeffrey Martin, Jeremy Cowan, Tom Walters, and Debra Inglis
Four potentially biodegradable mulch products (BioAgri, BioTelo, WeedGuardPlus, and SB-PLA-10) were evaluated during 2010 in three contrasting regions of the United States (Knoxville, TN; Lubbock, TX; and Mount Vernon, WA) and compared with black plastic mulch and a no-mulch control for durability, weed control, and impact on tomato yield in high tunnel and open field production systems. WeedGuardPlus, BioTelo, and BioAgri had the greatest number of rips, tears, and holes (RTH) and percent visually observed deterioration (PVD) at all three sites (P ≤ 0.05), and values were greater in the open field than high tunnels, likely as a result of high winds and greater solar radiation and rainfall. SB-PLA-10 showed essentially no deterioration at all three sites and was equivalent to black plastic in both high tunnels and the open field. Weed growth at the sites did not differ in high tunnels as compared with the open field (P > 0.05). Weed growth at Knoxville and Mount Vernon was greatest under SB-PLA-10 (P ≤ 0.02), likely as a result of the white, translucent nature of this test product. Tomato yield was greater in the high tunnels than open field at all three sites (P ≤ 0.03), except for total fruit weight at Knoxville (P ≤ 0.53). Total number of tomato fruit and total fruit weight were lowest for bare ground at both Knoxville (150 × 104 fruit/ha and 29 t·ha−1; P ≤ 0.04) and Mount Vernon (44 × 104 fruit/ha and 11 t·ha−1; P ≤ 0.008). At Knoxville, the other mulch treatments were statistically equivalent, whereas at Mount Vernon, BioAgri had among the highest yields (66 × 104 fruit/ha and 16 t·ha−1). There were no differences in tomato yield resulting from mulch type at Lubbock.
Melissa T. McClendon, Debra A. Inglis, Kevin E. McPhee, and Clarice J. Coyne
Dry pea (Pisum sativum L.) production in many areas of the world may be severely diminished by soil inhabiting pathogens such as Fusarium oxysporum f. sp. pisi race 1, the causal organism of fusarium wilt race 1. Our objective was to identify closely linked marker(s) to the fusarium wilt race 1 resistance gene (Fw) that could be used for marker assisted selection in applied pea breeding programs. Eighty recombinant inbred lines (RILs) from the cross of Green Arrow (resistant) and PI 179449 (susceptible) were developed through single-seed descent, and screened for disease reaction in race 1 infested field soil and the greenhouse using single-isolate inoculum. The RILs segregated 38 resistant and 42 susceptible fitting the expected 1:1 segregation ratio for a single dominant gene (χ2 = 0.200). Bulk segregant analysis (BSA) was used to screen 64 amplified fragment length polymorphism (AFLP) primer pairs and previously mapped random amplified polymorphic DNA (RAPD) primers to identify candidate markers. Eight AFLP primer pairs and 15 RAPD primers were used to screen the RIL mapping population and generate a linkage map. One AFLP marker, ACG:CAT_222, was within 1.4 cM of the Fw gene. Two other markers, AFLP marker ACC:CTG_159 at 2.6 cM linked to the susceptible allele, and RAPD marker Y15_1050 at 4.6 cM linked to the resistant allele, were also identified. The probability of correctly identifying resistant lines to fusarium wilt race 1, with DNA marker ACG:CAT_222, is 96% percent. These markers will be useful for marker assisted breeding in applied pea breeding programs.
Russell W. Wallace, Annette L. Wszelaki, Carol A. Miles, Jeremy S. Cowan, Jeffrey Martin, Jonathan Roozen, Babette Gundersen, and Debra A. Inglis
Field studies were conducted during 2010 and 2011 in Knoxville, TN; Lubbock, TX; and Mount Vernon, WA; to compare high tunnel and open-field organic production systems for season extension and adverse climate protection on lettuce (Lactuca sativa) yield and quality. The climates of these locations are diverse and can be typified as hot and humid (Knoxville), hot and dry (Lubbock), and cool and humid (Mount Vernon). In both years, 6-week-old lettuce seedlings of ‘New Red Fire’ and ‘Green Star’ (leafy type), ‘Adriana’ and ‘Ermosa’ (butterhead type), and ‘Coastal Star’ and ‘Jericho’ (romaine type) were transplanted in the late winter or early spring into subplots covered with black plastic and grown to maturity (43 to 65 days). Lettuce harvest in Knoxville occurred at 50 to 62 days after transplanting (DAT), with open-field lettuce harvested an average of 9 days earlier compared with high tunnel plots both years (P > 0.0001). The earlier than anticipated harvests in the open-field in Knoxville in 2010 were due to lettuce bolting. In Lubbock, high tunnel lettuce was harvested an average 16 days earlier in 2010 compared with open-field lettuce (P > 0.0001), while in 2011, high temperatures and bolting required that open-field lettuce be harvested 4 days earlier than lettuce grown in high tunnels. On average, lettuce cultivars at Mount Vernon matured and were harvested 56 to 61 DAT in 2010 and 54 to 64 DAT in 2011 with no significant differences between high tunnel and open-field production systems. Total and marketable yields at Mount Vernon and Lubbock averaged across cultivars were comparable in both high tunnel and open-field plots. At Knoxville, although total yields were significantly higher (P > 0.0062) in high tunnels than open-field plots, incidence of insect, disease, and physiological damage in high tunnel plots reduced lettuce quality and marketable yield (P > 0.0002). Lettuce head length:diameter ratio (LDR) averaged across cultivars was equal between high tunnel and the open field at all three locations. High tunnel production systems offer greater control of environments suitable for lettuce production, especially in climates like Knoxville and Lubbock where later-planted open-field systems may be more susceptible to temperature swings that may affect lettuce quality. These results suggest that although high tunnel culture alone may influence lettuce yield and quality, regional climates likely play a critical role in determining the impact of these two production systems on marketable lettuce yields.