Kale (Brassica oleracea L.) ranks highest among vegetable crops for lutein and beta-carotene carotenoids, which function as antioxidants in disease prevention. Nitrogen (N) rate and N form influence plant growth and alter pigment composition and accumulation. The objectives of these experiments were to investigate the effect of N rate and form on biomass and accumulation of plant pigments in the leaf tissues of kale. Three kale cultivars were grown using nutrient solution culture. In the first study, N treatment rates were 6, 13, 26, 52, and 105 mg·L–1, at a constant NH4-N:NO3-N ratio. Kale biomass increased linearly in response to increasing N rate. On a fresh weight basis, lutein and beta-carotene were not affected by N rate. However, carotenoids calculated on a dry weight basis increased linearly in response to increasing N rate. In a second study, kale was grown under: 100% NH4-N:0% NO3-N, 75% NH4-N:25% NO3-N, 50% NH4-N:50% NO3-N, 25% NH4-N:75% NO3-N, and 0% NH4-N:100% NO3-N, at a N rate of 105 mg·L–1. Linear increases in biomass were observed for each kale cultivar as percentage of NO3-N increased. Lutein concentrations increased 155%, 73%, and 39% for `Toscano', `Winterbor', and `Redbor' kale, respectively, as N form changed 0% NO3-N to 100% NO3-N. Concentration of leaf beta-carotene increased linearly in response to increasing NO3-N in each cultivar tested. Nitrogen management should be considered in crop production programs designed to increase the concentrations of nutritionally important carotenoids.