Petunia × hybrida Villm. `Red Flash' plants received either 10 or 20 mol·day-1 photosynthetic photon flux (PPF) in growth chambers at: 175 μmol·m-2·s-1 for 16 hours, 350 μmol·m-2·s-1 for 8 or 16 hours, or 350 μmol·m-2 s-1 for 8 hours plus 8 hours of incandescent photoperiod extension (5 μmol·m-2·s-1 PPF). The irradiation components of peak, total, and duration were examined. Doubling total PPF increased total carbohydrate (CHO) production by 60%, seedling dry weight (DW) by 30%, rate of seedling growth by 25%, and acid invertase activity by 50% compared to the other treatments, once the seedlings had reached the two-leaf stage. Seedlings receiving 20 mol·day-1 PPF partitioned 14% more CHO into ethanol soluble sugars rather than starch, which may explain the increase in relative growth rate observed with supplemental irradiance treatments. Extending the photoperiod for 8 hours with 5 μmol·m-2·s-1 PPF reduced total CHO production by 50% compared to the same treatment without photoperiodic lighting. Treatment with 350 μmol·m-2·s-1 for 8 hours resulted in the highest O2 evolution (8.8 μmol O2/min per dm2). Increasing the photoperiod from 8 to 16 hours gave the lowest rate of O2 evolution (4.5 μmol O2/min per dm2). Previous reports of the importance of photosynthetic period in controlling partitioning between starch and sugars may have simply observed a decreasing rate of starch accumulation due to increased total PPF.
David F. Graper and Will Healy
David F. Graper and Will Healy
The increase in photosynthetic photon flux (PPF) and plant temperature associated with supplemental high pressure sodium (HPS) irradiation were investigated during Petunia × hybrids Villm. `Red Flash' seedling development. Seedlings were treated for 14 days following emergence or 5 days after the first true leaf had expanded to 1 mm. Treatments consisted of continuous infrared (IR) radiation (Ambient + IR), ambient conditions with spill-over radiation from adjacent treatments (Ambient - IR), root zone heating to 19.5C (RZ Heat), continuous HPS irradiation at 167 μmol·s-1.m-2 PPF (HPS + IR) or continuous HPS irradiation at 167 μmol-1·m-2 PPF filtered through a water bath to remove IR (HPS - IR). Linear regression of natural log-transformed fresh weights indicated that increasing ambient PPF 53% and elevating plant temperature 4.3C (HPS + IR) increased seedling relative growth rate (RGR) by 45% compared with the control (Ambient - IR). Elevating plant temperature with + IR by 4.8C without supplementing PPF (Ambient + IR) increased RGR by 31% but failed to increase fresh weight (FW) above controls and resulted in etiolated plants that were unsuitable for transplanting. Once plants were removed from supplemental treatment and returned to ambient conditions, RGR for all treatments was similar. The increased FW promoted by IR and HPS treatments was maintained for up to 7 days after treatment. Therefore, the increased seedling growth responses observed with HPS treatment were due primarily to an increase in RGR during HPS treatment that is not sustained beyond treatment.